S100A8 Monoclonal Antibody

Code: CSB-MA0206411A0m

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Size:

50μg100μg

US$210

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Species Reactivity: Human

Raised in: mouse

Application: ELISA,IHC

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Target Background

Product Details

Uniprot NO.

P05109

Target Names

S100A8

Alternative Names

60B8Ag antibody; AI323541 antibody; B8Ag antibody; BEE11 antibody; CAGA antibody; Calgranulin-A antibody; Calprotectin L1L subunit antibody; Calprotectin; included antibody; CFAG antibody; CGLA antibody; Chemotactic cytokine CP-10 antibody; CP-10 antibody; Cystic fibrosis antigen antibody; L1Ag antibody; Leukocyte L1 complex light chain antibody; MA387 antibody; MIF antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; Myeloid-related protein 8 antibody; Neutrophil cytosolic 7 kDa protein antibody; NIF antibody; p8 antibody; Pro-inflammatory S100 cytokine antibody; Protein S100-A8 antibody; S100 calcium binding protein A8 (calgranulin A) antibody; S100 calcium binding protein A8 antibody; S100 calcium-binding protein A8 antibody; S100A8 antibody; S100A8/S100A9 complex; included antibody; S10A8_HUMAN antibody; Urinary stone protein band A antibody

Raised in

mouse

Species Reactivity

Human

Immunogen

Recombinant Human S100A8

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

monoclonal

Isotype

IgG1

Clone No.

1A1C1

Purification Method

>95%,protein G purifed

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA,IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:500

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunohistochemical of paraffin-embedded human cervical cancer using CSB-MA0206411A0m at dilution of 1:200
Immunohistochemical of paraffin-embedded human spleen using CSB-MA0206411A0m at dilution of 1:200

Description

The S100A8 monoclonal antibody was synthesized by immunizing mice with purified recombinant human S100A8 protein and then isolating B cells from the mouse spleen. These B cells were then fused with myeloma cells to create hybridomas, which were screened to select those that produced the S100A8 antibody. The selected hybridomas were inoculated into the mouse abdominal cavity. The resulting S100A8 monoclonal antibody was purified from the mouse ascites using protein G affinity chromatography. Its purity is over 95%. It is recommended for the detection of the human S100A8 protein in ELISA and IHC.

S100A8 protein is involved in the modulation of immune responses and inflammation, and wound healing. It also possesses antimicrobial activity.

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Target Background

Function(From Uniprot)

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.; (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.

Gene References into Functions

Study found that that the co-stimulation with both advanced glycation end-products and Porphyromonas gingivalis LPS increased S100A8 and S100A9 mRNA expression in gingival epithelial cells. These results demonstrated that the upregulation of calprotectin in gingival epithelial cells may primarily function intracellularly in this tissue and may contribute to the pathological process of periodontitis. PMID:28771806
Our results showed that S100A8/A9, absent or present at very low level in skin biopsies from healthy subjects, is dramatically upregulated in each epidermal layer from psoriatic patients. PMID:29605872
The results suggest that the calcium-binding proteins S100A8 and S100A9 in myeloid cells have an immune regulatory effect. PMID:29933628
Median MRP8/14 levels were higher in patients with Takayasu arteritis. PMID:29196802
S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes. PMID:29595187
Wide intra- and interindividual differences in calprotectin concentrations in the meconium may reflect intestinal inflammation associated with the fetal adaptation to life outside the uterus. PMID:28463850
Salivary calprotectin levels were significantly higher in RAS [Recurrent aphthous stomatitis] group (23.72 +/- 4.28 mg/L) compared to the HC [Healthy Controls] (21.59 +/- 4.27 mg/L) (P = 0.013). No significant relationship was found between calprotectin levels and age or gender in both groups (P >0.05). PMID:29519972
the levels of S100A8 and S100A9 in the sera, and calprotectin levels in the sera and urines of bladder cancer patients, were determined. PMID:28609200
MRP-8/14 concentration increases in coronary artery blood in association with thrombus formation in acute coronary syndrome. PMID:28986057
ANGPTL6, S100A8 and S100A9 are overexpressed in skin of psoriasis patients versus zero expression in controls. PMID:27698489
Elevated breast cancer cell and stromal S1008 protein expression are significant indicators of poorer outcomes in early stage breast cancer patients. Evaluation of S100A8 protein expression may provide additional prognostic information beyond traditional breast cancer prognostic biomarkers. PMID:28717852
S100A8 was required for laser-induced new collagen synthesis in skin cells. PMID:29270708
S100A8 methylation has a role in diagnosis and prognosis of hepatocellular carcinoma PMID:27462864
This study demonstrated that S100A8 was detected in the serums of Alzheimer disease patients PMID:27911324
Studies indicate S100A8 as the most overexpressed gene, and the cell cycle pathway as the most promising biomarker and therapeutic target for relapsed childhood B-acute lymphoblastic leukemia (B-ALL). PMID:28183295
Low S100A8 expression is associated with head and neck neoplasms. PMID:26883112
Report role of fecal calprotectin assay in the diagnosis and management of inflammatory bowel disease. PMID:28735301
S100A8 and S100A9 aggravate Coxsackievirus B3-induced myocarditis and might serve as therapeutic targets in inflammatory cardiomyopathies. PMID:29158436
Data suggest that fecal calprotectin may be a potential biomarker to identify patients with ankylosing spondylitis (AS) at risk of developing inflammatory bowel disease (IBD). PMID:28148281
Elevated S100A8 and S100A9 gene expression in SP-infected HMEECs and in the middle ear mucosa of OM, minor co-localized with neutrophil markers suggests that middle ear epithelial cell secretion of S100A8 and S100A9 may play a role in the pathogenesis of recurrent and chronic OM PMID:28964306
results of this study support an additional role of calprotectin in assessing inflammatory activity in patients with RA PMID:28832684
Data show that E3 ubiquitin ligase HRD1 (HRD1) decreased the protein level of S100A8 through ubiquitination. PMID:28423597
High S100A8 expression is associated with glioblastoma. PMID:27006175
This study implicates S100A8 and S100A9 as important mediators of tumor cell aggressiveness, and highlights the therapeutic potential of S100A8 and S100A9 for interference of metastasis. PMID:27086923
Data indicate a statistical correlation between fecal calprotectin (FC) and gastrointestinal graft-versus-host disease (GvHD). PMID:28293713
Perinatal alarmins S100A8 and S100A9 specifically altered MyD88-dependent proinflammatory gene programs. S100 programming prevented hyperinflammatory responses without impairing pathogen defense. TRIF-adaptor-dependent regulatory genes remained unaffected by perinatal S100 programming and responded strongly to lipopolysaccharide, but were barely expressed. PMID:28459433
Expression of S100A8, S100A9 and S100A12 is modulated by eicosapentaenoic acid and docosahexaenoic acid during Inflammation in adipose tissue and mononuclear cells. PMID:28125622
High S100A8 expression is associated with lung metastasis in malignant melanoma. PMID:27151304
Data show that the standardized nerve growth factor (NGF) concentration was negatively correlated with continuous pain, neuropathic pain and total score, and the standardized S100 proteins S100A8/A9 concentration was positively correlated with present pain intensity and continuous pain. PMID:27936243
S100A8 and S100A9 proteins in concentrations secreted by CD34(-)/CD31(+) circulating angiogenic cells (CACs) with impaired function reduce endothelial cell capillary-like network formation. These effects appear to be mediated by Toll-like receptor 4 and are absent with S100A8 and S100A9 in concentrations secreted by healthy CD34(-)/CD31(+) CACs. PMID:27793853
MRP8 elevated serum levels in patients with Behcet's disease indicate MRP8 role in the pathogenetic mechanisms of Behcet's disease. PMID:25471894
High baseline serum MRP8/14 levels are associated with lower survival in Chinese peritoneal dialysis patients. PMID:22699162
In acute coronary syndromes, MRP8 and MRP8/14 complex are specific ligands of TLR4, which induce the release of TNFalpha and probably other pro-inflammatory agents from monocytes PMID:21782178
study revealed a novel role of MRP8/14 in the autophagy-mediated elimination of intracellular BCG by promoting reactive oxygen species generation PMID:25312864
MRP8 has a crucial role in stimulating IL-6 expression by rheumatoid arthritis fibroblast-like synoviocytes. PMID:23619188
decreased pretreatment levels of myeloid-related protein-8/14 and C-reactive protein may have a role in poor adherence to treatment with tumor necrosis factor inhibitors in juvenile idiopathic arthritis PMID:27562034
MRP8/14 can activate cells by binding to TLR4, leading to the release of proinflammatory cytokines. These mediators can lead to increased disease activity in enthesitis-related arthritis. PMID:24231443
Fibronectin adhesive capacity and integrin expression of monocytes of type 1 and type 2 diabetic patients is related to the subjects' serum levels of MRP-8. PMID:15277376
Excessive release of cytotoxic MRP8/MRP14 by activated phagocytes might therefore present an important molecular pathomechanism contributing to endothelial damage during vasculitis and other inflammatory diseases PMID:17095618
MRP8 expression is related to the pathological stage of laryngeal squamous cell carcinoma. PMID:22256739
MRP8/14 in ankylosing spondylitis is associated with peripheral arthritis and active disease PMID:27738838
Circulating MRP-8/14 is associated with TX-dependent platelet activation in ACS, even during low-dose aspirin treatment, suggesting a contribution of residual TX to MRP-8/14 shedding, which may further amplify platelet activation. PMID:25037196
Phosphorylation inhibits tubulin polymerization in microtubules. PMID:15331440
Data suggest that tissue infiltrating macrophages secreting MRP8/14 may contribute to myositis, by driving the local production of cytokines directly from muscle. PMID:24286299
calprotectin induces IL-6 and MCP-1 production in Human Gingival Fibroblasts via TLR4 signaling that involves MAPK and NF-kappaB, resulting in the progression of periodontitis. PMID:27925202
Findings suggest that S100A8/A9 may be involved in the inflammatory response with induction of proinflammatory cytokines and may serve as a clinicopathological marker for disease activity in AOSD. PMID:27537874
Peripheral blood levels of S100A8 and TRAF6 in SAE patients were elevated and might be related to the severity of SAE and predict the outcome of SAE. PMID:27411454
Low S100A8/A9 levels may contribute to poor wound healing in colonized chronic wounds with striking difference between diabetic foot ulcers and venous leg ulcers. PMID:27084691
Suggest that, in individuals with lichen planus, S100A8 may exert distinct immunomodulatory and cytotoxicity functions. PMID:26632637
Serum calprotectin was significantly higher in rheumatoid arthritis patients than controls, and was correlated with disease activity. It was higher in seropositive than in seronegative patients. PMID:26767827

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Subcellular Location

Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway.

Protein Families

S-100 family

Tissue Specificity

Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes

Database Links

HGNC: 10498

UNIGENE: Hs.416073

KEGG: hsa:6279

STRING: 9606.ENSP00000357721

OMIM: 123885

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