CD44 Monoclonal Antibody

Code: CSB-MA004938A0m

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Raised in: Mouse

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P16070

Target Names

CD44

Alternative Names

LHR antibody; BA-1 antibody; CD 44 antibody; CD44 antibody; CD44 antigen antibody; CD44 molecule (Indian blood group) antibody; CD44 molecule antibody; CD44_HUMAN antibody; CDw44 antibody; CDW44 antigen antibody; Cell surface glycoprotein CD44 antibody; chondroitin sulfate proteoglycan 8 antibody; CSPG8 antibody; ECMR-III antibody; Epican antibody; Extracellular matrix receptor III antibody; GP90 lymphocyte homing/adhesion receptor antibody; HCELL antibody; hematopoietic cell E- and L-selectin ligand antibody; Heparan sulfate proteoglycan antibody; Hermes antigen antibody; homing function and Indian blood group system antibody; HSA antibody; HUTCH-I antibody; HUTCH1 antibody; HUTCHI antibody; Hyaluronate receptor antibody; IN antibody; INLU-related p80 Glycoprotein antibody; MC56 antibody; MDU2 antibody; MDU3 antibody; MGC10468 antibody; MIC4 antibody; MUTCH I antibody; MUTCH1 antibody; PGP-1 antibody; PGP-I antibody; PGP1 antibody; Phagocytic glycoprotein 1 antibody; Phagocytic glycoprotein I antibody; Soluble CD44 antibody

Raised in

Mouse

Species Reactivity

Human

Immunogen

Recombinant Human CD44 antigen protein (21-220AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1, Igk

Clone No.

6G2D2

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:40000
IHC	1:50-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate
All lanes: CD44 antibody at 1:1500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa
Observed band size: 95 kDa
Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: CD44 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa
Observed band size: 95 kDa
IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of MCF-7 cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay histogram showing Hela cells stained with CSB-MA004938A0m (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Description

This CD44 monoclonal antibody was produced against a Recombinant Human CD44 antigen protein in order to bind to CD44 antigens. Fusion of cell culture myeloma cells with mouse spleen cells antibodies resulted in hybrid cells which produced large amounts of CD44 monoclonal antibody. The cell fusion resulted in the cells with two properties, one with the ability to grow continually, and the other with the ability to produce the CD44 monoclonal antibody, which has been validated in ELISA, WB, IHC, IF, FC.

CD44 belongs to the family of adhesion factors and is a type of transmembrane single-chain glycoprotein widely expressed on the cell surface. Originally described as a granulocyte-T lymphocyte antigen, it was named the CD44 cluster at the third international working group meeting of leukocyte differentiation antigens. CD44 is a family of glycoproteins encoded by a single gene located on chromosome 11p13. The molecular mass is between 80 and 200 kDa. CD44 is highly glycosylated, including N-linked and O-linked glycosylation and chondroitin sulfate. Heparinized.
CD44 is an important type of adhesion factor, which is widely distributed on the surface of lymphocytes, monocytes, endothelial cells and other cells. The diversity of CD44 ligands determines the diversity of its functions.
CD44 functions as a collector of cytokines and proteases, and plays an important role in organogenesis, hematogenesis, inflammation, and autoimmune processes. As a serious disease threatening human life, tumors have received more and more attention. Early diagnosis can buy enough time for better treatment.

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Target Background

Function(From Uniprot)

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion.

Gene References into Functions

Interleukin-4 induces a CD44high /CD49bhigh PC3 subpopulation with tumor-initiating characteristics. PMID:29236307
we demonstrated that miR711mediated downregulation of CD44 expression inhibited EMT of gastric cancer cells in vitro and in vivo by downregulating vimentin protein expression and upregulating Ecadherin protein expression through transfection, qRTPCR and western blotting. PMID:30226620
Expression levels of MACC1, CD44, Twist1, and KiSS-1 are related to duration of overall survival among patients with colonic adenocarcinoma. PMID:30021598
miR-218-5p was downregulated in invasion front cells and negatively regulates oral squamous cell carcinoma invasiveness by targeting the CD44-ROCK pathway. PMID:29990854
our study demonstrated that miR-520b inhibits the malignancy of HNC through regulation of cancer stemness conversion by targeting CD44. PMID:28515423
The work identified two new host factors that may act as receptors for P. falciparum during invasion: CD44 and CD55. (Review) PMID:29249333
Reports indicate that high CD44 expression in ascites tumor cells (ATC) correlates with CSC and EMT phenotype, both regulated by the tumor microenvironment through several signaling pathways, including the TGF-beta signaling pathway. PMID:30142697
Data identified Twist1 and CD44 as novel REST targeted genes and provide new insight into the epigenetic regulation of Twist1 and CD44 by REST. PMID:28256535
Data show increased CD44 levels in ovarian cancer patient samples correlated with enhanced expression of the mesenchymal spliced variant CD44s (standard) and a concurrent decrease in the epithelial variants (CD44v). Moreover, CD44s was upregulated upon TGFbeta1-induced EMT, which was mediated through the downregulation of the splicing factor, ESRP1. Overexpression of CD44s induced EMT, invasion, and chemoresistance. PMID:29130517
The current evidence suggests that CD44 is an efficient prognostic factor in pancreatic cancer. PMID:29683068
the CD44-NRF2 axis might be a promising therapeutic target for the control of stress resistance and survival of CD44(high) CSC population within breast tumors. PMID:29729523
These results reveal a novel positive feedback loop involving CD44S and YAP1, in which CD44S functions as both an upstream regulator and a downstream effector of YAP1 in hepatocellular carcinoma. PMID:29649630
CHI3L1 expression is a novel biomarker for the prognosis of gastric cancer and these findings have thus identified CHI3L1/CD44 axis as a vital pathway and potential therapeutic target in gastric cancer . PMID:30165890
The selective binding of HA-CH-NP/siRNA to CD44-positive tumor endothelial cells. PMID:29890852
Results showed that the expression of IGF1R appears to be highly correlated with the expression of ABCG2 in osteosarcoma and with the expression of CD44 in osteosarcoma patients under age of 10. PMID:29892839
CD44 Polymorphisms are associated with Gastric Cancer. PMID:29802692
verification of the presence of stem cell-like cells in the epithelial component through the immunopositivity to Oct-4 and CD44 in benign odontogenic lesions of variable biological behaviors PMID:29971493
The genotypes CT, CT+TT, TT, and allele T in rs13347 of CD44 may be risk factors for breast cancer. PMID:29748526
Peripheral blood lymphocyte subsets in patients with lung cancer are different from those in healthy people, and circulating CD44+ and CD54+ lymphocytes seem to be a promising criterion to predict survival in lung cancer patients undergoing chemotherapy PMID:29148014
The minority of cancer stem cells would not be detected by immunohistochemistry using panCD44. PMID:29682524
CD44 regulated TLR2 responses in human macrophages, whereby a reduction in CD44 levels or engagement of CD44 by its ligand (HA) or a CD44-specific Ab reduced NF-kappaB translocation and downstream proinflammatory cytokine production. PMID:29196459
Our results suggested that CD44 expression could be used as a marker for the prediction of gastric cancer development, particularly in patients with precancerous gastric lesions carrying AG or GG, who were selected to surveillance follow-up for gastric cancer prevention. PMID:29445738
CD44 polymorphism rs13347 acts as a risk factor for cancer, especially in Chinese, while the minor allele of polymorphism rs11821102 may be associated with a decreased susceptibility to cancer PMID:28000766
ntermediate Molecular Mass Hyaluronan and CD44 interactions on PMNs potently elicit F-actin cytoskeleton polymerization and p38- and ERK1/2-MAPK phosphorylation to enhance PMN function. PMID:28730511
CD44v9 in tumor specimens has potential as a novel indicator for identifying a cisplatin-chemoresistant population among urothelial cancer patients. CD44v8-10 contributes to reactive oxygen species defenses, which are involved in chemoresistance, by promoting the function of xCT, which adjusts the synthesis of glutathione. PMID:29385995
CD44 standard isoform was especially upregulated after high-dose X-ray irradiation. PMID:29106581
Upregulated miR-373 levels and simultaneously downregulated levels of CD44 and E-cadherin were noted in this study. PMID:29307338
MiRNA-34a suppresses invasion and metastatic in esophageal squamous cell carcinoma by regulating CD44. PMID:29094237
This study using syngeneic mouse models, which better model the disease in humans than conventional xenografts, suggests that NIR-PIT with anti-CD44-IR700 is a potential candidate for the treatment of Oral cavity squamous cell carcinoma PMID:28923838
miR214 represses endogenous CD44 expression by targeting the 3'untranslated region in HeLa, Raji and Jurkat cells. PMID:29138813
This analysis indicated that corilagin is mainly involved in the glycolysis pathway. Seahorse XF96 extracellular acidification rate analysis confirmed that corilagin inhibited glycolysis by downregulation of CD44 and STAT3 PMID:28791374
an important role for HYAL2 in CD44 alternative splicing. PMID:29162741
CD146 suppresses BC progression as a target of CD44-downstream signaling PMID:29121955
based on our data, the markers CD44 and CD24 do not reflect the features of CSC and unfavorable prognosis and do not clarify the role and clinical significance of the immunophenotype CD44+/CD24-. PMID:28967636
CD44s levels correlate with EGFR signature and predict poor prognosis in glioblastomas. PMID:28716909
This study demonstrated that expression of CD44S and CD44 splice variants CD44V3, CD44V6, and CD44V10 was significantly higher in AD patients compared to non-AD controls PMID:28550248
Network analysis of overlapping genes revealed the effects on tubulins (Tubb2a, Tubb3, Tubb4b), Nfe2l2, S100a4, Cd44, and Nfkb2, all of which are linked to TBI-relevant outcomes, including epileptogenesis and tissue repair PMID:27530814
In obese patients, hepatic CD44 was strongly upregulated in NASH patients (p=0.0008) and correlated with NAFLD activity score (NAS) (p=0.001), ballooning (p=0.003), alanine transaminase (p=0.005) and hepatic CCL2 (p<0.001) and macrophage marker CD68 (p<0.001) expression. Correction of NASH was associated with a strong decrease in liver CD44(+) cells. PMID:28323124
These findings suggest that CD44v and CD44s cells play differently important roles in the progression and metastasis of GBC and the isoform switch triggers epithelial-mesenchymal transition PMID:28677740
Results suggest significant role of CD44 variants (rs13347, rs187115 and rs11821102) in modulating individual's cancer susceptibility in Asians (meta-analysis). PMID:27521214
CD44v9 may be a good biomarker for prognosis prediction and for chemoprevention or biomarker-driven therapies only for Early Gastric Cancer, and it appeared to be associated with lymph node metastasis PMID:25779358
Tunicamycin inhibited CD44s overexpression-associated cell migration. PMID:29377347
Osteopontin and CD44 play important roles in the development and progression of meningioma and can be used as prognostic markers for tumor recurrence and progression as well as therapeutic targets for the development of new drugs. PMID:29504367
CD24 and CD44 are upregulated in human pancreatic cancer compared to chronic pancreatitis and may be related to the development of pancreatic cancer. PMID:28659655
our study results showed that CD44v6 is an important regulator of GC tumorigenesis, angiogenesis, and survival in an IL-6 mediated, pSTAT3-dependent manner PMID:28507278
expressed by rheumatoid synovial fibroblasts in a MIF allele-dependent fashion and undergoes functional regulation and activation by autocrine/paracrine MIF PMID:27872288
Circulating tumor cells expressing cytokeratin and tumor-initiating cell markers, including ALDH, CD133, and CD44, were identified in patients with pancreatic adenocarcinoma. These TIC-like CTCs were associated with poor prognosis after surgical resection and with an increased incidence of tumor recurrence PMID:27789528
High CD44 expression is associated with renal cell carcinoma. PMID:27588469
FAM83D promotes HCC recurrence by promoting CD44 expression and CD44+ CSCs malignancy. PMID:27769048
Intraperitoneal enrichment of cancer stem-like cells, from ovarian cancer cell lines or primary ovarian tumor, provides a rational approach for cancer stem-like cell isolation and characterization using CD44 and prominin-1(CD133) as selection markers. PMID:27655682

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus.

Tissue Specificity

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

Database Links

HGNC: 1681

UNIGENE: Hs.502328

KEGG: hsa:960

STRING: 9606.ENSP00000398632

OMIM: 107269

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