Abbreviation
Recombinant Rhesus macaque IL5 protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of >2.5x105 IU/mg.
Alternative Names
IL5Interleukin-5; IL-5; Eosinophil differentiation factor; T-cell replacing factor; TRF
Species
Macaca mulatta (Rhesus macaque)
Expression Region
20-134aa
Target Protein Sequence
IPTEIPASAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYIG GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Interleukin-5 is a key eosinophil-lineage cytokine whose cross-reactive signaling in nonhuman primate models makes this rhesus macaque IL-5 particularly valuable for preclinical studies of allergic inflammation and asthma. This tag-free recombinant protein, spanning residues 20–134 of the mature sequence, demonstrates potent bioactivity with an ED50 below 4 ng/ml in TF-1 cell proliferation assays, corresponding to a specific activity exceeding 2.5 × 10⁵ IU/mg—sufficient to drive robust JAK/STAT signaling pathway studies and eosinophil differentiation experiments. Endotoxin levels below 1.0 EU/μg ensure that downstream immune cell responses in proliferation assays, in vivo primate inflammation models, and antibody development campaigns reflect genuine IL-5 signaling rather than LPS-driven artifacts. Purity exceeding 98% by SDS-PAGE, combined with this stringent endotoxin control, supports use as a reference standard in cytokine detection assays and as a reliable coating antigen for ELISA-based antibody validation.
