Abbreviation
Recombinant Rhesus macaque IL4 protein (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Alternative Names
IL4Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
Species
Macaca mulatta (Rhesus macaque)
Expression Region
25-153aa
Target Protein Sequence
HNCHIALREI IETLNSLTEQ KTLCTKLTIT DILAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE DFLERLKTIM REKYSKCSS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered 2 × PBS, pH 7.4, 5 % trehalose
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-4 is a pleiotropic cytokine central to Th2 polarization, B-cell class switching, and alternative macrophage activation, making a well-characterized rhesus macaque ortholog essential for translational immunology in nonhuman primate models. This tag-free recombinant protein, encompassing the full-length mature sequence (residues 25–153) produced in *E. coli*, demonstrates potent bioactivity with an ED₅₀ below 1.0 ng/mL in a TF-1 cell proliferation assay, corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg — providing a reliable basis for dose-dependent functional studies including T-cell and B-cell activation, Th2 differentiation, and JAK/STAT signaling pathway analysis. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell readouts, which supports use in sensitive primary cell cultures and *in vivo* primate studies of inflammation or infection. Purity exceeding 96% by SDS-PAGE further qualifies this protein as a reference standard in cytokine detection assays and as a positive control for antibody validation by ELISA or Western blot.
