Abbreviation
Recombinant Rhesus macaque IL1A protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 10 pg/ml, corresponding to a specific activity of >1.0x108 IU/mg.
Alternative Names
IL1A; Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1
Species
Macaca mulatta (Rhesus macaque)
Expression Region
113-271aa
Target Protein Sequence
SAPFSFLSNM TYHFIRIIKH EFILNDTLNQ TIIRANDQHL TAAAIHNLDE AVKFDMGAYT SSKDDTKVPV ILRISKTQLY VSAQDEDQPV LLKEMPEINK TITGSETNFL FFWETHGTKN YFISVAHPNL FIATKHDNWV CLAKGLPSIT DFQILENQA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-1α is a potent upstream mediator of innate immune signaling in rhesus macaque models, making species-matched reagents essential for translational studies of NF-κB and MAPK pathway activation. This tag-free recombinant protein encompasses the full mature sequence (residues 113–271) and demonstrates exceptional signaling potency, with an ED50 below 10 pg/mL in a murine D10S proliferation assay, corresponding to a specific activity exceeding 1.0 × 10⁸ IU/mg. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts, which supports use in sensitive immune cell functional assays such as T-cell proliferation, macrophage activation, and downstream phospho-signaling studies, as well as in vivo primate inflammation models where confounding endotoxin effects would compromise data interpretation. Purity exceeding 97% by SDS-PAGE further provides a suitable basis for applications including ELISA standard curves, antibody validation, and cytokine reference standardization.
