Abbreviation
Recombinant Mouse Il1b protein (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of >5.0x108 IU/mg.
Alternative Names
Il1bInterleukin-1 beta; IL-1 beta
Species
Mus musculus (Mouse)
Expression Region
118-269aa
Target Protein Sequence
VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 ?m filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Interleukin-1 beta is a master regulator of innate immune signaling, driving NF-κB and MAPK pathway activation with broad relevance to inflammation, fever, and host defense models. This tag-free recombinant mouse IL-1β, encompassing the full mature sequence (residues 118–269), demonstrates exceptional signaling potency with an ED50 below 2 pg/ml in a murine D10S proliferation assay, corresponding to a specific activity exceeding 5.0 × 10⁸ IU/mg — providing a reliable reference standard for cytokine detection assays and a potent stimulus for cell-based functional studies including T-cell activation, macrophage differentiation, and NF-κB signaling experiments. Endotoxin levels below 1.0 EU/μg ensure that downstream immune cell responses reflect genuine IL-1β signaling rather than LPS-driven artifacts, which is critical for in vivo murine inflammation models and for generating validated antibodies where antigenic specificity must be unambiguous. Purity exceeding 96% by SDS-PAGE further supports use as a coating antigen in ELISA development and as a positive control in western blot workflows.
