Abbreviation
Recombinant Human IL20 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x106 IU/mg.
Alternative Names
Cytokine Zcyto10; Four alpha helix cytokine; IL-20; IL10D; IL20; IL20_HUMAN; Interleukin 20; Interleukin-20; MGC96907; UNQ852/PRO1801; ZCYTO10
Species
Homo sapiens (Human)
Expression Region
25-176aa
Target Protein Sequence
M+LKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.2, with trehalose
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-20 signals through heterodimeric receptor complexes to regulate epithelial innate immunity and inflammatory tissue remodeling, making precise recombinant forms essential for dissecting its biology. This tag-free human IL-20, encompassing the full mature sequence (residues 25–176), demonstrates an ED50 below 0.5 ng/ml in a proliferation assay using IL-20Rα/IL-20Rβ co-transfected BaF3 cells, corresponding to a specific activity exceeding 2.0 × 10⁶ IU/mg — potency that supports use in JAK/STAT and MAPK pathway activation studies, keratinocyte or monocyte functional assays, and as a quantitative reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts, providing a suitable basis for in vivo inflammation models and primary immune cell experiments where confounding innate immune activation must be excluded. Purity above 95% by SDS-PAGE, combined with this endotoxin profile, also satisfies the criteria typical for antibody development workflows including immunization, ELISA coating, and Western blot controls.
