Abbreviation
Recombinant Human IL17F protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of >5.0x104 IU/mg.
Alternative Names
CANDF6; Cytokine ML 1; Cytokine ML-1; IL 17F; IL 24; IL-17F; IL-24; Il17f; IL17F_HUMAN; Interleukin 17F; Interleukin 24; Interleukin-17F; Interleukin-24; ML 1; ML1; Mutant IL 17F; OTTHUMP00000016602
Species
Homo sapiens (Human)
Expression Region
31-163aa
Target Protein Sequence
M+RKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-17F is a key mediator of mucosal immunity and chronic inflammatory signaling, driving neutrophil recruitment and pro-inflammatory gene expression through NF-κB and MAPK pathway activation. This tag-free recombinant human IL-17F, encompassing the full mature sequence (residues 31–163), demonstrates robust signaling potency with an ED50 below 20 ng/mL in an IL-6 secretion assay using murine NIH/3T3 cells, corresponding to a specific activity exceeding 5.0 × 10⁴ IU/mg — providing a reliable basis for cell-based functional assays, NF-κB/MAPK signaling studies, and use as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts, making this protein appropriate for sensitive immune cell activation experiments, in vivo inflammation models, and antibody development workflows including ELISA coating and positive controls, while purity above 95% by SDS-PAGE further supports consistent performance across these demanding applications.
