Abbreviation
Recombinant Human IL10 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Alternative Names
CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL-10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin-10; MGC126450; MGC126451; T-cell growth inhibitory factor; TGIF
Species
Homo sapiens (Human)
Expression Region
19-178aa
Target Protein Sequence
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-10 is a pleiotropic anti-inflammatory cytokine central to immune homeostasis, and this tag-free recombinant form covering the full mature sequence (residues 19–178) preserves native homodimer assembly critical for faithful STAT3 signaling studies. In MC/9-2 cell proliferation assays, the protein demonstrates an ED50 below 1 ng/mL—corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg—which provides a suitable basis for dose-dependent functional work including T-cell suppression assays, macrophage polarization experiments, and JAK/STAT pathway activation studies. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that would otherwise confound cytokine-specific readouts in primary immune cell cultures, making this preparation appropriate for in vivo inflammation models and as a quantitative reference standard in ELISA-based cytokine detection. Produced in *E. coli* at greater than 95% purity by SDS-PAGE, this protein also supports antibody validation workflows including immunogen use and positive control applications in Western blot.
