Abbreviation
Recombinant Horse IL1B protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 20 pg/ml, corresponding to a specific activity of >5.0x107 IU/mg.
Alternative Names
IL1BInterleukin-1 beta; IL-1 beta
Species
Equus caballus (Horse)
Expression Region
116-268aa
Target Protein Sequence
AAMHSVNCRL RDIYHKSLVL SGACELQAVH LNGENTNQQV VFCMSFVQGE EETDKIPVAL GLKEKNLYLS CGMKDGKPTL QLETVDPNTY PKRKMEKRFV FNKMEIKGNV EFESAMYPNW YISTSQAEKS PVFLGNTRGG RDITDFIMEI TSA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 0.1 % Tween-80
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-1β is a master pro-inflammatory cytokine that drives NF-κB and MAPK signaling cascades, and this recombinant equine form delivers exceptional potency with an ED50 below 20 pg/ml in a D10S cell proliferation assay, corresponding to a specific activity exceeding 5.0 × 10⁷ IU/mg. The tag-free, mature-sequence protein (residues 116–268) produced in *E. coli* faithfully represents the native processed cytokine, making it a suitable basis for equine-specific immune cell activation studies, NF-κB signaling pathway dissection, and use as a reference standard in species-matched cytokine detection assays. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that would otherwise confound innate immune readouts, which supports use in sensitive functional assays such as primary cell proliferation, macrophage activation, and antibody validation by ELISA. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, satisfies the quality criteria typical for both *in vitro* mechanistic studies and *in vivo* equine inflammation models.
