Abbreviation
Recombinant Rat Prl protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Research Area
Signal Transduction
Alternative Names
Prl; Prolactin; PRL
Species
Rattus norvegicus (Rat)
Expression Region
30-226aa
Target Protein Sequence
LPVCSGGDCQ TPLPELFDRV VMLSHYIHTL YTDMFIEFDK QYVQDREFIA KAINDCPTSS LATPEDKEQA QKVPPEVLLN LILSLVHSWN DPLFQLITGL GGIHEAPDAI ISRAKEIEEQ NKRLLEGIEK IISQAYPEAK GNEIYLVWSQ LPSLQGVDEE SKDLAFYNNI RCLRRDSHKV DNYLKFLRCQ IVHKNNC
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Prolactin drives JAK2/STAT5 signaling through the prolactin receptor, making validated bioactivity essential for meaningful downstream results. This tag-free recombinant rat Prl, spanning residues 30–226 of the mature protein, demonstrates an ED50 below 1.0 ng/ml in Nb2-11 cell proliferation assays—corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg—confirming its suitability for receptor binding assays, STAT5 phosphorylation studies, and cell-based signaling experiments such as cAMP induction. Endotoxin levels below 1.0 EU/μg, combined with purity greater than 98% by SDS-PAGE, satisfy the criteria typically required for in vivo pharmacology models and antibody development programs where contaminating signals must be minimized. Researchers can confidently employ this protein as an immunoassay calibration standard or in structure-function studies exploring prolactin receptor engagement.
