Abbreviation
Recombinant Human PTN protein (Active)
Purity
>96% as determined by SDSPAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been pre-coated with 100 µl/well of a solution of 5-10 µg/ml rHuPTN.
Alternative Names
HARP; HB-GAM; HBBM; HBGAM; HBGF-8; HBGF8; HBNF; HBNF-1; HBNF1; heparin affin regulatory protein; Heparin binding growth associated molecule; Heparin binding growth factor 8; Heparin binding neurite outgrowth promoting factor 1; Heparin-binding brain mitogen; Heparin-binding growth factor 8; Heparin-binding growth-associated molecule; heparin-binding neurite outgrowth promoting factor; Heparin-binding neurite outgrowth-promoting factor 1; NEGF1; Neurite growth promoting factor 1; Neurite outgrowth-promoting factor; heparin-binding; OSF-1; OSF1; Osteoblast-specific factor 1; pleiotrophin (heparin binding growth factor 8; neurite growth-promoting factor 1); Pleiotrophin; PTN; PTN_HUMAN
Species
Homo sapiens (Human)
Expression Region
33-168aa
Target Protein Sequence
GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 m filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Pleiotrophin is a heparin-binding growth factor with well-established roles in neurite outgrowth, neuronal survival, and angiogenesis, making it a critical tool for neurodevelopmental and tumor biology research. This tag-free recombinant human PTN covers the full mature sequence (residues 33–168) and has been validated for biological activity by demonstrating enhanced neurite outgrowth in E16–E18 rat embryonic cortical neurons at coating concentrations of 5–10 µg/ml, confirming its suitability for neuronal differentiation studies, cell survival assays, and receptor-ligand binding experiments targeting RPTPβ/ζ via ELISA or SPR. Produced in *E. coli*, the protein lacks glycosylation—consistent with the natively unglycosylated character of PTN—which provides a homogeneous preparation appropriate for quantitative binding assays and use as an ELISA standard. Purity exceeding 96% by SDS-PAGE combined with endotoxin levels below 1.0 EU/µg satisfies the criteria typically required for sensitive primary neuron cultures and in vivo tumor biology models.
