Abbreviation
Recombinant Human NRN1 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by by a cell proliferation assay using rat C6 cells is less than 25 ng/ml, corresponding to a specific activity of >4.0x104 IU/mg.
Research Area
Cell Biology
Alternative Names
Neuritin 1; Neuritin; NRN; Nrn1; NRN1_HUMAN; OTTHUMP00000015989; RP3 380B8.2 protein
Species
Homo sapiens (Human)
Expression Region
28-115aa
Target Protein Sequence
AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Neuritin (NRN1) is a GPI-anchored neurotrophic factor that promotes neurite outgrowth and synaptic plasticity, making it a key target in neuronal survival and differentiation research. This tag-free recombinant human NRN1 fragment (residues 28–115) encompasses the mature secreted domain and demonstrates robust biological potency, with an ED50 below 25 ng/mL in a rat C6 cell proliferation assay, corresponding to a specific activity exceeding 4.0 × 10⁴ IU/mg. The E. coli expression system provides a non-glycosylated protein suitable for receptor-ligand binding studies via SPR or ELISA, where the absence of variable glycan modifications supports more reproducible quantitative readouts. Purity above 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based proliferation and survival assays, neuronal differentiation studies, and use as a calibration standard in antibody characterization workflows.
