Abbreviation
Recombinant Staphylococcus phage 42D sak protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 5.0x104 IU/mg.
Research Area
Cell Biology
Alternative Names
sak; Staphylokinase; Sak42D
Species
Staphylococcus phage 42D (Bacteriophage P42D)
Expression Region
28-163aa
Target Protein Sequence
SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDGKRNELL SPRYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Staphylokinase activates plasminogen into plasmin through a unique cofactor mechanism distinct from mammalian plasminogen activators, making it a valuable tool for studying fibrinolytic pathways and thrombolytic drug development. This tag-free recombinant form, covering residues 28–163 of the mature protein, preserves an unobstructed active site and demonstrates a specific activity of 5.0 × 10⁴ IU/mg as measured by fibrin lysis in an agarose plate assay—providing a quantitative benchmark suitable for kinetic parameter analysis (Km, Vmax, kcat), inhibitor screening with IC50 determination, and use as a positive control in plasminogen activation assays. The absence of fusion tags eliminates steric interference that could confound substrate specificity profiling or crystallographic studies. Purity exceeding 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for accurate enzyme kinetics and cell-based fibrinolysis experiments where endotoxin contamination would compromise results.
