Abbreviation
Recombinant Human PSPN protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TT medullary thyroid cancer cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
Research Area
Neuroscience?
Alternative Names
PSPN; Persephin; PSP
Species
Homo sapiens (Human)
Expression Region
61-156aa
Target Protein Sequence
ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Persephin signals through the GFRα4/RET receptor complex to promote neuronal survival, yet its recombinant production demands careful attention to the disulfide-rich cystine knot fold. This tag-free human PSPN fragment (aa 61–156) encompasses the mature, receptor-binding domain and demonstrates an ED50 below 10 ng/mL in TT medullary thyroid cancer cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg. Such potency supports use in neuronal survival and proliferation assays, RET-dependent signaling studies, and receptor-ligand binding experiments via SPR or ELISA. Produced in *E. coli*—appropriate for this non-glycosylated growth factor—the protein meets purity greater than 97% by SDS-PAGE with endotoxin levels below 1.0 EU/μg, satisfying the quality criteria typical for cell-based bioassays and in vivo regenerative neuroscience models.
