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Recombinant Human Persephin protein (PSPN), partial (Active)

Recombinant Human Persephin protein (PSPN), partial (Active)

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Code: CSB-AP002951HU

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US$142

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Express system: E.Coli

Species: Homo sapiens (Human)

Tag Info: Tag-Free

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Target Background

Product Details

Abbreviation

Recombinant Human PSPN protein, partial (Active)

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TT medullary thyroid cancer cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x10⁵ IU/mg.

Uniprot NO.

O60542

Research Area

Neuroscience?

Alternative Names

PSPN; Persephin; PSP

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

61-156aa

Target Protein Sequence

ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

10.3 kDa

Protein Length

Partial

Tag Info

Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Lead Time

5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

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Description

Persephin signals through the GFRα4/RET receptor complex to promote neuronal survival, yet its recombinant production demands careful attention to the disulfide-rich cystine knot fold. This tag-free human PSPN fragment (aa 61–156) encompasses the mature, receptor-binding domain and demonstrates an ED50 below 10 ng/mL in TT medullary thyroid cancer cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg. Such potency supports use in neuronal survival and proliferation assays, RET-dependent signaling studies, and receptor-ligand binding experiments via SPR or ELISA. Produced in *E. coli*—appropriate for this non-glycosylated growth factor—the protein meets purity greater than 97% by SDS-PAGE with endotoxin levels below 1.0 EU/μg, satisfying the quality criteria typical for cell-based bioassays and in vivo regenerative neuroscience models.

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Target Background

Function(From Uniprot)

Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.

Gene References into Functions

Results suggested that PSPN is a possible key regulator of oral squamous cell carcinoma (OSCC) progression via PSPN-RET-mitogen-activated protein kinase activation and that PSPN overexpression may have diagnostic potential for OSCC. PMID:24375483
Results identify persephin, a GDNF family member, as a novel ligand for GFRalpha1/RET receptor complex. PMID:20350599
Persephin/GFRalpha4 is unable to recruit RET protein into lipid rafts. PMID:15225646
The results obtained suggest the involvement of NTN, PSP, and ART in processes subserving both the organization of this cortical region during development and the functional activity and maintenance of the mature human hippocampal neurons. PMID:15829225
No differences were found in the allelic frequencies of the variants or in the haplotype distribution between Hirschsprung's disease patients & controls, nor to any demographic/clinical parameters within the group of patients. PMID:18970938