Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

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Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

Name: Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E06994Rb

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Code: CSB-E06994Rb

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

IGF1 ELISA Kit; IGF-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit

Abbreviation

IGF1

Uniprot NO.

Q95222

Species

Oryctolagus cuniculus (Rabbit)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	80-100
1:2	Average %	100
	Range %	91-105
1:4	Average %	98
	Range %	92-115
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of rabbit IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	90	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.715	2.725	2.720	2.605
5	2.035	2.002	2.019	1.904
2.5	0.989	1.078	1.034	0.919
1.25	0.591	0.602	0.597	0.482
0.625	0.342	0.352	0.347	0.232
0.312	0.201	0.212	0.207	0.092
0.156	0.175	0.185	0.180	0.065
0	0.111	0.119	0.115

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Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

Description

This Rabbit IGF1 ELISA Kit was designed for the quantitative measurement of Rabbit IGF1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.

Gene References into Functions

Data suggest caloric restriction modifies response of ovarian granulosa cells to leptin on cell proliferation/apoptosis, MAP kinase signaling, and release of hormones (estradiol and IGFI). The caloric restriction studies were conducted in rabbits; then, isolated rabbit granulosa cells were exposed to recombinant human leptin. (IGF1 = insulin-like growth factor I) PMID:27894742
Intraplacental gene therapy with Ad-IGF-1 corrects naturally occurring rabbit model of intrauterine growth restriction. PMID:25738403
Our studies demonstrate that the active SVCT2 is expressed in IVD cells and that the expression of this transporter is regulated by growth factors IGF-1 and dexamethasone. PMID:23415019
increased endogenous IGF1 and IGF2 expression by the blastocyst compensates for the loss of systemic insulin and IGF. PMID:22465205
IGF1 showed a significantly higher capacity to form the posterior mesoderm and primitive streak (stage 2 and 3) than blastocysts cultured without growth factors PMID:21827825
Overall, IGF-1 promoted atherosclerosis by affecting endothelial function and aging. PMID:21219916