Rabbit C-Reactive Protein,CRP ELISA Kit

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Name: Rabbit C-Reactive Protein,CRP ELISA Kit
Brand: CUSABIO
SKU: CSB-E06847Rb

CSB-E06847Rb

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma

Detect Range: 7.8 pg/mL-2000 pg/mL

Sensitivity: 2 pg/mL

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Alternative Names

CRP ELISA kit; PTX1C-reactive protein ELISA kit

Abbreviation

CRP

Uniprot NO.

P02742

Species

Oryctolagus cuniculus (Rabbit)

Sample Type

serum, plasma

Detection Range

7.8 pg/mL-2000 pg/mL

Sensitivity

2 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	90-102
1:2000	Average %	91
	Range %	82-96
1:4000	Average %	90
	Range %	85-98
1:8000	Average %	94
	Range %	83-98

Recovery

The recovery of rabbit CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	91	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.196	0.198	0.197
500	0.353	0.371	0.362
125	0.669	0.651	0.660
31.3	1.084	1.035	1.060
7.8	2.018	1.974	1.996
0	2.913	3.122	3.018

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Datasheet & COA

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Rabbit C-Reactive Protein,CRP ELISA Kit

Description

This Rabbit CRP ELISA Kit was designed for the quantitative measurement of Rabbit CRP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-2000 pg/mL and the sensitivity is 2 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Gene References into Functions

Rabbit CRP acute-phase system binds phosphocholine and phosphoethanolamine. PMID:23600950
IL-6 augments mechanical strain-induced CRP synthesis in vessels via the stretch-activated channel-NF-kappaB pathway. PMID:23257175
CRP antagonism of eNOS is mediated by coupling of FcgammaRI to FcgammaRIIB by Src kinase and activation of inositol 5'-phosphatase 1, and consistent with this mechanism, both FcgammaRI and FcgammaRIIB are required for CRP to blunt endothelial repair in vivo. PMID:21940940
Metformin attenuated the progression of aortic atherosclerosis via down-regulation of serum C-reactive protein. PMID:20108078
During abdominal aortic aneurysm formation, increased serum hsCRP levels derive from aneurysmal arteries with degenerating elastic lamina. PMID:19502992