Sheep Interleukin-8,IL-8 ELISA Kit

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Code: CSB-E13202Sh

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 6.25 pg/mL-400 pg/mL

Sensitivity: 1.56 pg/mL

Trial Size:

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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit

Abbreviation

CXCL8

Uniprot NO.

P36925

Species

Ovis aries (Sheep)

Sample Type

serum, plasma, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-90

1:2

Average %

Range %

80-95

1:4

Average %

Range %

87-99

1:8

Average %

Range %

90-105

Recovery

The recovery of sheep IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-100
EDTA plasma (n=4)	96	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.312	2.231	2.272	2.094
200	1.753	1.854	1.804	1.626
100	1.289	1.339	1.314	1.136
50	0.824	0.887	0.856	0.678
25	0.558	0.597	0.578	0.400
12.5	0.492	0.472	0.482	0.304
6.25	0.308	0.297	0.303	0.125
0	0.184	0.171	0.178

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Sheep IL8 ELISA Kit was designed for the quantitative measurement of Sheep IL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Customer Reviews and Q&A

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Citations

Cerebral and lung effects of a new generation synthetic surfactant with SP┞ and SP┟ analogs in preterm lambs Carmen Rey-Santano PhD.et al,/,2017
Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics N Nasreldin,/,2020
Construction and Biological Characteristics of a Quadruple Gene-Deleted Strain of Orf Virus as a Vaccine Candidate J Zhang, R Xin, J Zhao, R Wu, D Su, M Li, Y Zhu,/,2025

Target Background

Function(From Uniprot)

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Gene References into Functions

In response to C. abortus infection, both ovine and bovine turbinate cells produce CXCL8 mRNA and protein late in the bacterial developmental cycle. PMID:26342452
IL-8 signaling does not mediate lipopolysaccharide-induced lung inflammation and lung maturation. PMID:19574422

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

UNIGENE: Oar.485

KEGG: oas:443418

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