Sheep Interleukin 10,IL-10 ELISA Kit

Product Details

Customer Reviews and Q&A

Citations

Target Background

Name: Sheep Interleukin 10,IL-10 ELISA Kit
Brand: CUSABIO
SKU: CSB-E12817Sh

Promotion

Code: CSB-E12817Sh

Rate （5.0）
Review （1）
Citation （10）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 5 pg/mL-80 pg/mL

Sensitivity: 5 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Target Name

interleukin 10

Alternative Names

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

Abbreviation

IL10

Uniprot NO.

Q29408

Species

Ovis aries (Sheep)

Sample Type

serum, plasma, tissue homogenates

Detection Range

5 pg/mL-80 pg/mL

Sensitivity

5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	98
	Range %	93-104
1:4	Average %	84
	Range %	82-88
1:8	Average %	90
	Range %	85-97

Recovery

The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
80	0.202	0.196	0.199
40	0.365	0.345	0.355
20	0.623	0.641	0.632
10	1.147	1.168	1.158
5	2.344	2.189	2.267

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Images

Sheep Interleukin 10,IL-10 ELISA Kit

Description

Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that plays an important role in immune regulation and homeostasis. Regulatory T cells, macrophages, and B cells produce IL-10 primarily. IL-10 works to suppress inflammatory responses by blocking pro-inflammatory cytokine production and reducing antigen presentation. This immunosuppressive activity makes IL-10 necessary for preventing excessive immune activation. It also helps maintain tissue integrity during inflammatory processes. Research into IL-10 levels offers valuable insights into immune system function, inflammatory disease progression, and therapeutic monitoring in various pathological conditions.

The Sheep Interleukin 10 ELISA Kit (CSB-E12817Sh) measures IL-10 quantitatively in sheep samples using a competitive assay principle. This kit works with serum, plasma, and tissue homogenates. The detection range spans 5 pg/mL to 80 pg/mL with a sensitivity of 5 pg/mL. The assay needs 50-100 μL sample volume and takes 1-5 hours to complete. Detection occurs at 450 nm wavelength, giving researchers a reliable tool for IL-10 quantification in Ovis aries specimens.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

[ERROR] Response ended prematurely

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:5:"sheep";}}

Sample type: a:1:{i:0;a:2:{i:0;s:17:"Biological fluids";i:1;s:14:"synovial fluid";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used the CSB ELISA IL10 kit to detect IL-10 in synovial fluid of sheep. I tried it on normal samples, and osteoarthritis samples. I used a multiskan, with automatic conversion of absorbance, after automatic analysis (four parameter logistic curve-fit). I did not have a multichannel pipet so my values are not perfectly repeatable. The kit is easy to use, and protocol is clear. Just keep in mind that you have to read the plates at 450nm and correct with a 620-nm read. It is actually mentioned in the “other supplies required” section, but it is not remind in the “assay procedure” section, “assay procedure summary” section or in the “calculation of result” section (so during the assay there is a risk to forget the 2nd read).

By Anonymous

Effects of maternal undernutrition during late gestation and/or lactation on colostrum synthesis and immunological parameters in the offspring S. Chadio.et al,/,2014
Ambient particulate air pollution and circulating antioxidant enzymes: A repeated-measure study in healthy adults in Beijing, China Shaowei Wu. et al,/,2015
Relationship between Pro-Inflammatory Cytokines, IL-10 Anti-Inflammatory Cytokine and Serum Proteins in Healthy Lambs and with Diarrhea Fernndez A.et al,/,2016
Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,/,2016
Avaliação da resposta imune de ovinos infectados com Haemunchus contortus. BERNE, N, et al,/,/
Effect of progesterone on the vaccination and immune response against Chlamydia abortus in sheep A.Murcia-Belmonte, et al,/,2019
Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle Herman A P, et al,/,2019
Peripheral and placental immune responses in sheep after experimental infection with Toxoplasma gondii at the three terms of gestation Castano P, et al,/,2019
Sheep immune‐stimulated with Saccharomyces boulardii show reduced prolificacy of Haemonchus contortus NB Pinheiro,/,2022
Construction and Biological Characteristics of a Quadruple Gene-Deleted Strain of Orf Virus as a Vaccine Candidate J Zhang, R Xin, J Zhao, R Wu, D Su, M Li, Y Zhu,/,2025

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.

Gene References into Functions

The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID:25817420
Significant differences in mitogen-specific lymphocyte proliferation in vitro and in expression of TGF-beta1 and IL-10 genes in hepatic lymph nodes were observed at acute and chronic phases of infection, respectively. PMID:19751473

Hide All

Subcellular Location

Secreted.

Protein Families

IL-10 family

Database Links

UNIGENE: Oar.528

KEGG: oas:443342

Promotions