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Rat soluble receptor activator of nuclear factor-kB ligand,sRANKL ELISA Kit

Rat soluble receptor activator of nuclear factor-kB ligand,sRANKL ELISA Kit

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Code: CSB-E05126r

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, cell culture supernates

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Product Details

Target Name

tumor necrosis factor (ligand) superfamily, member 11

Alternative Names

Tnfsf11 ELISA Kit; Opgl ELISA Kit; Rankl ELISA Kit; Trance ELISA Kit; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; Osteoclast differentiation factor ELISA Kit; ODF ELISA Kit; Osteoprotegerin ligand ELISA Kit; OPGL ELISA Kit; Receptor activator of nuclear factor kappa-B ligand ELISA Kit; RANKL ELISA Kit; TNF-related activation-induced cytokine ELISA Kit; TRANCE ELISA Kit; CD antigen CD254) [Cleaved into: Tumor necrosis factor ligand superfamily member 11 ELISA Kit; membrane form; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; soluble form] ELISA Kit

Abbreviation

TNFSF11

Uniprot NO.

Q9ESE2

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

Recovery

Typical Data

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

Tumor necrosis factor superfamily member 11 (TNFSF11), commonly known as RANKL, is a cytokine that regulates bone metabolism and immune system function. As a member of the TNF superfamily, TNFSF11 plays a central role in osteoclast differentiation and activation, making it a key mediator of bone resorption processes. Various cell types release the soluble form of this receptor activator of nuclear factor-κB ligand. It serves as an important biomarker in bone-related diseases and inflammatory conditions.

This quantitative sandwich ELISA kit measures rat soluble RANKL in serum, plasma, and cell culture supernatants with a detection range of 62.5 pg/mL to 4000 pg/mL and sensitivity of 15.6 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for quantifying TNFSF11 levels in Rattus norvegicus samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining bone metabolism and remodeling processes. Studies have applied the kit to measure RANKL levels in serum samples as part of comprehensive bone marker profiling, alongside other bone formation and resorption indicators.

• Orthodontic tooth movement research - Measuring RANKL levels during orthodontic treatment to understand bone remodeling mechanisms
• Bone metabolism studies - Quantifying RANKL as a key regulator of osteoclast activity in conjunction with other bone turnover markers
• Serum biomarker profiling - Measuring circulating RANKL levels to evaluate bone resorption processes in experimental models

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Citations

Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone /,/,2012
Effect of whole body vibration therapy on circulating serotonin levels in an ovariectomized rat model of osteoporosis Wei QS et al,/,2014
Biomarkers for Bisphosphonate-related Osteonecrosis of the Jaw Kim JW et al,/,2015
CP-25, a novel compound, protects against autoimmune arthritis by modulating immune mediators of inflammation and bone damage Chang Y.et al,/,2016
Antioxidative peptide from milk exhibits antiosteopenic effects through inhibition of oxidative damage and bone-resorbing cytokines in ovariectomized rats Sanusi BelloMadaPh.D.et al,/,2017
The impact of forced swimming on expression of RANKL and OPG in a type 2 diabetes mellitus rat model Pezhman LPezhman L.et al,/,2018
Evaluation of the osteoprotective potential of whey derived-antioxidative (YVEEL) and angiotensin-converting enzyme inhibitory (YLLF) bioactive peptides in ovariectomised rats Masum Pandey.et al,/,2018
Antiosteopenic Effect of Buffalo Milk Casein-Derived Peptide (NAVPITPTL) in Ovariectomized Rats Srinu Reddi.et al,/,2018
Cocoa administration may accelerate orthodontic tooth movement by inducing osteoclastogenesis in rats Ananto Alhasyimi, et al,/,2018
Postorthodontic Relapse Prevention by Administration of Grape Seed (Vitis vinifera) Extract Containing Cyanidine in Rats Alhasyimi AA,/,2019
Differential skeletal response in adult and aged rats to independent and combinatorial stimulation with pulsed electromagnetic fields and mechanical vibration Cai J,/,2020
Effects of C-peptide replacement therapy on bone microarchitecture parameters in streptozotocin-diabetic rats. S Maurotti,/,2020
Ziziphus spina-christi leaf extract attenuates mercuric chloride-induced liver injury in male rats via inhibition of oxidative damage SS Ramadan,/,2021
Evaluation of the Ameliorative Effect of Zinc Nanoparticles against Silver Nanoparticle–Induced Toxicity in Liver and Kidney of Rats AM Shehata,/,2021
Investigating the therapeutic potential of sinomenine in rheumatoid arthritis: anti-inflammatory, antioxidant, and immunomodulatory mechanisms J Li,/,2023
Multi-omics and network pharmacology reveal Huayu-Tongbi decoction reduced arthritis-related bone erosion B Chen, L Yang, H Wang, P Yu, M Ma, M Chen,/,2025
Zinc alleviates vascular calcification by activating ERK1/2-mediated autophagy G Guo, J Long, T Xu, X Li, D Sui, X Feng, L Yao,/,2025

Target Background

Function(From Uniprot)

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.

Gene References into Functions

results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME PMID:29297549
in this animal model the increase of RANK/RANKL and HGF markers is related to a specific immune response, and probably contributed to the evolution of periodontal disease PMID:29211121
In the supplemented and nonsupplemented rats groups, RANKL was upregulated compared with the control group. PMID:28473060
the canonical Wnt pathway inhibitor DKK1 blocked the osteogenesis effect and rescued the ratio of RANKL/OPG in periodontal ligament stem cells under force treatment for 1h PMID:27154288
study identified a number of differentially expressed bone-related mRNAs of potential significance and confirmed the osteoprotegerin/receptor activator of nuclear factor kappa-B (RANK)/RANK ligand (RANKL) pathway PMID:25406873
High-dose diosgenin treatment down-regulated expression of RANKL significantly in tibia from OVX rats compared to control. PMID:25257532
Data indicate that after loading jump (JL) for 6 weeks, the expressions of of interleukin 6 (IL-6), and ligand of receptor activator of NF-kappaB (RANKL) genes were markedly elevated in tibia. PMID:25200151
mechanical stimulation inhibits the activity of RANKL in rats PMID:23553492
Our findings indicate that the expression of RANKL in the occlusal portion of the bony crypt is unrelated to osteoclast recruitment and differentiation but is crucial to their activation during the creation of the eruption pathway. PMID:23636419
RANK and RANKL were expressed by T lymphocytes and macrophages in acute cellular kidney rejection after transplantation in rats PMID:23769040
In rheumatoid arthritis methotrexate/leflunomide combination therapy would relive the synovium hypertrophy through depressing cell viability and osteoclasia through decreasing RANKL and Il-17. PMID:23334376
There was no change in OPN expression after orthodontic retention and bone remodeling. PMID:22353912
the increase in RANK-RANKL expression is a response to podocyte injury, and RANK-RANKL may be a novel receptor-ligand complex for the survival response during podocyte injury. PMID:22848465
Insulin could promote osteoblastic differentiation of calcifying vascular smooth muscle cells by increased RANKL expression through ERK1/2 activation, but not PI3K/Akt activation. PMID:22194983
TRANCE/RANKL is constituively expressed by rat plasmacytoid dendritic cells. PMID:22428075
Pressure-overloaded myocardium generates RANKL, which induces inflammation mediators and myocardial inflammation. PMID:22298642
Bone loss and recovery in a receptor activator for nuclear factor kappa B ligand (RANKL)-administered rat model was assessed PMID:20480144
RANKL/OPG are key factors linking bone formation to resorption during bone remodeling. PMID:21771583
Vitamin C deficiency increased osteoclastogenesis by increasing RANK expression. PMID:20444587
These results suggest that antibody to RANKL can inhibit A. actinomycetemcomitans-specific T cell-induced periodontal bone resorption by blockade and reduction of tissue sRANKL. PMID:21078845
RANKL contributes to vascular calcification by regulating bone morphogenetic protein-2 and calcification inhibitor matrix Gla protein (MGP) expression, as well as bone-related proteins, and is counteracted by estrogen in a receptor-dependent manner. PMID:20595654
the toothless (tl) osteopetrotic rat mutation is not in the TNFSF11 locus PMID:11804028
accelerates nuclear translocation of nuclear factor kappa B in osteoclasts by elevaing cytosolic Ca2+ PMID:12496256
Effects of 12-O-tetradecanoylphorbol-13-acetate on osteoclastogenesis and its role in RANKL-induced signaling. PMID:14672351
RANK ligand is strongly up-regulated during acute heart allograft rejection; its blockade prolongs rat heart allograft survival. PMID:14734743
Detection of RANKL mRNA and protein in bone cells. PMID:15704000
Results suggest that the RANKL expressed in thymic epithelial cells plays a role in the development of T cells during thymic regeneration. PMID:15844004
Portasystemic shunting caused low turnover osteoporosis that was RANKL independent. PMID:16874862
Prolactin decreased RANKL mrna in cultured osteoblasts. PMID:18432284
These data indicate that serum 25-hydroxyvitamin D levels are a major determinant of osteoclastogenesis and bone mineral volume. PMID:18597628
Recombinant RANKL (rRANKL) mutants within the TNF-like core domain exhibited diminished osteoclastogenic potential as compared with wild-type rRANKL1 encoding the full TNF-like core domain [amino acids (aa) 160-318]. PMID:19008464
Orchiectomy increases the concentration of free sRANKL in bone marrow of aged rats. PMID:19501680
Bony spur formation can thus be considered a process that occurs independent of TNFalpha and RANKL and is triggered by destructive arthritis. PMID:19714640