Rat placenta growth factor,PLGF ELISA kit

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Name: Rat placenta growth factor,PLGF ELISA kit
Brand: CUSABIO
SKU: CSB-E07400r

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Code: CSB-E07400r

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Citation （6）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 3.12 pg/mL-200 pg/mL

Sensitivity: 0.78 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

Pgf ELISA Kit; Plgf ELISA Kit; Placenta growth factor ELISA Kit; PlGF ELISA Kit

Abbreviation

PGF

Uniprot NO.

Q63434

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PLGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	94
	Range %	89-98
1:4	Average %	93
	Range %	87-97
1:8	Average %	99
	Range %	93-102

Recovery

The recovery of rat PLGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	94	91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.246	2.234	2.240	2.085
100	1.472	1.456	1.464	1.309
50	0.901	0.914	0.908	0.753
25	0.639	0.649	0.644	0.489
12.5	0.364	0.389	0.377	0.222
6.25	0.300	0.308	0.304	0.149
3.12	0.252	0.242	0.247	0.092
0	0.156	0.154	0.155

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat placenta growth factor(PLGF)ELISA Kit allows for the in vitro quantitative determination of PLGF concentrations in serum, plasma, or tissue homogenates. The detection mechanism of this kit is based on the Sandwich-ELISA technique. PLGFin the sample is bound to the anti-rat PLGFantibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled PLGFantibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with PLGFcontent in the sample. This kit exclusively recognizes rat PLGF. The kit has been quality controlled with high sensitivity, strong specificity, good linearity, precision of less than 10%, recovery of 89%-98%, and high lot-to-lot consistency. Refer to the product manual for more information.

PLGF is a member of the VEGF family and is mainly expressed in the placenta. It is a crucial molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. Specifically, PLGF plays a role in trophoblast growth and differentiation. In human atherosclerotic lesions, PLGF expression is linked to plaque inflammation and neovascular growth. PLGF is also a potential biomarker for pre-eclampsia, a pregnancy complication characterized by hypertension and proteinuria in pregnancy.

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The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an l-NAME model of pre-eclampsia Sooraj Baijnath.et al,/,2016
Ferulic acid alleviates symptoms of preeclampsia in rats by upregulating vascular endothelial growth factor Gong W.et al,/,2017
Fructose Consumption Affects Placental Production of H2S: Impact on Preeclampsia-Related Parameters X Zhao,/,2024
Celastrol attenuates symptoms of preeclampsia in rats by inhibiting matrix metalloproteinase-9 Sha Xiao.et al,/,2018
Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein Wen-Haur Chao.et al,/,2018
The involvement of long noncoding RNA APOA1-AS in the pathogenesis of preeclampsia HY Yang,/,2023

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth.

Gene References into Functions

Studied the influence of dendrobium nobile Lindley (DNL) extract and its bibenzyl component moscatilin on the expression of placental growth factor (PLGF) and Norrie disease protein (NDP) in hypoxia model in retinal cells. Found DNL and moscatilin are able to protect against retinal ischemic/hypoxic changes respectively by downregulating PLGF and upregulating NDP. PMID:29933759
this paper show that knockdown of PLGF mitigates damage in hyperoxic lung by blocking of NFkappaB signaling PMID:27280587
PLGF triggers focal adhesion disassembly in alveolar type II cells via inhibiting the activation of focal adhesion kinase. PMID:25515701
PlGF had a positive feedback regulation with 15-lipoxygenase expression and 15-hydroxyeicosatetraenoic acid generation. PMID:24001991
placental gene expression is modulated by gestational conditions that might disrupt the fetal growth PMID:23691181
PlGF repairs myocardial ischemia through mechanisms of angiogenesis, cardioprotection and recruitment of myo-angiogenic competent marrow progenitors PMID:21969865
augmentation of brain Placental growth factor is associated with development of epilepsy. PMID:22079325
PLGF expression in the lung tissue in rats with paraquat-induced pulmonary fibrosis is upregulated. PMID:17997886
This is the first demonstration that sustained intraocular PGF production induces vascular and retinal changes similar to those observed in the early stages of diabetic retinopathy. PMID:21408222
Intramyocardial injections of autologous myoblasts overexpressing placental growth factor improved cardiac function, attenuated adverse cardiac remodeling, induced angiogenesis, and probably enhanced survival of grafted myoblasts. PMID:21329947
This study shows for the first time the placental growth factor upregulation in regenerating livers, which is related to hypoxia stimulation and liver growth. PMID:21088600
Endothelial cell-released H2O2 acts as a positive regulator of placenta growth factor gene and protein expression in vascular smooth muscle cells. PMID:21123739
The ARPE19 human junctions in culture are modulated by VEGF through VEGFR-1 but not through VEGFR-2. PMID:18421240
The levels of PlGF-2 in non-malformed embryos of diabetic gestation and their placentas were significantly (P < 0.05) lower than the average of controls. PMID:19048427