Rat lipolysaccharide binding protein,LBP ELISA Kit

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Rat lipolysaccharide binding protein,LBP ELISA Kit

Name: Rat lipolysaccharide binding protein,LBP ELISA Kit
Brand: CUSABIO
SKU: CSB-E11184r

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Code: CSB-E11184r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 62.5 pg/mL-4000 pg/mL

Sensitivity: 15.6 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit

Abbreviation

LBP

Uniprot NO.

Q63313

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	81-89
1:10	Average %	94
	Range %	91-98
1:20	Average %	91
	Range %	85-97
1:40	Average %	86
	Range %	82-89

Recovery

The recovery of rat LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.869	2.899	2.884	2.688
2000	2.231	2.268	2.250	2.054
1000	1.514	1.569	1.542	1.346
500	0.969	0.999	0.984	0.788
250	0.628	0.648	0.638	0.442
125	0.432	0.469	0.451	0.255
62.5	0.297	0.278	0.288	0.092
0	0.197	0.195	0.196

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Rat lipolysaccharide binding protein,LBP ELISA Kit

Description

This Rat LBP ELISA Kit was designed for the quantitative measurement of Rat LBP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.

Subcellular Location

Secreted. Cytoplasmic granule membrane.

Protein Families

BPI/LBP/Plunc superfamily, BPI/LBP family

Tissue Specificity

Detected in lung, liver, heart and kidney.

Database Links

UNIGENE: Rn.48863

STRING: 10116.ENSRNOP00000019787

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