Rat leukemia inhibitory factor,LIF ELISA Kit

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Code: CSB-E07432r
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
Detect Range: 0.312 ng/mL-20 ng/mL
Sensitivity: 0.078 ng/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
Abbreviation
LIF
Uniprot NO.
Species
Rattus norvegicus (Rat)
Sample Type
serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %84-95
1:2Average %93
Range %89-97
1:4Average %99
Range %93-104
1:8Average %90
Range %86-98
Recovery
The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-92
EDTA plasma (n=4)9589-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.249 2.174 2.212 2.039
101.583 1.561 1.572 1.399
51.012 0.952 0.982 0.809
2.50.599 0.583 0.591 0.418
1.250.446 0.416 0.431 0.258
0.6250.320 0.299 0.310 0.137
0.3120.258 0.245 0.252 0.079
00.177 0.169 0.173  
Troubleshooting and FAQs
Description

This Rat LIF ELISA Kit was designed for the quantitative measurement of Rat LIF protein in serum, plasma, bronchoalveolar lavage fluid, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subcellular Location
Secreted.
Protein Families
LIF/OSM family
Database Links
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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