Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

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Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

Name: Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit
Brand: CUSABIO
SKU: CSB-E13027r

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Code: CSB-E13027r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell lysates

Detect Range: 1.56 ng/mL-100 ng/mL

Sensitivity: 0.39 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

Fabp4Fatty acid-binding protein ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; ALBP ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; A-FABP ELISA Kit; AFABP ELISA Kit; Fatty acid-binding protein 4 ELISA Kit

Abbreviation

FABP4

Uniprot NO.

P70623

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates, cell lysates

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-100
1:2	Average %	85
	Range %	80-90
1:4	Average %	89
	Range %	84-99
1:8	Average %	92
	Range %	86-101

Recovery

The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	90	82-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	1.974	1.913	1.944	1.817
50	1.721	1.681	1.701	1.574
25	1.416	1.311	1.364	1.237
12.5	0.958	0.897	0.928	0.801
6.25	0.669	0.629	0.649	0.522
3.12	0.395	0.364	0.380	0.253
1.56	0.217	0.207	0.212	0.085
0	0.131	0.123	0.127

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The product CSB-E13027r is a sandwich ELISA kit developed to measure concentrations of rat FABP4 in serum, plasma, cell lysates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of FABP4 in samples. The color intensity is measured at 450 nm via a microplate reader.

FABP4, also known as A-FABP or aP2, is mainly expressed in adipocytes and functions as a lipid chaperone protein. It regulates lipolysis and adipogenesis through interactions with hormone-sensitive lipase (HSL) and peroxisome PPAR-γ, respectively. FABP4 plays an important role in the development of insulin resistance and atherosclerosis in association with metaflammation. An increase in circulating FABP4 concentrations is linked to obesity, insulin resistance, diabetes, hypertension, cardiac dysfunction, and atherosclerosis. Reducing FABP4 levels or activity is associated with improved metabolic health.

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In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland Martinez-Arguelles D et al,/,2014

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Calycin superfamily, Fatty-acid binding protein (FABP) family

Database Links

UNIGENE: Rn.4258

STRING: 10116.ENSRNOP00000014701

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