Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit

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Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit

Name: Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit
Brand: CUSABIO
SKU: CSB-EQ027319RA

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Code: CSB-EQ027319RA

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma,tissue homogenates

Detect Range: 0.94 ng/mL-60 ng/mL

Sensitivity: 0.23 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

Krt18 ELISA Kit; Krt1-18Keratin ELISA Kit; type I cytoskeletal 18 ELISA Kit; Cytokeratin-18 ELISA Kit; CK-18 ELISA Kit; Keratin-18 ELISA Kit; K18 ELISA Kit

Abbreviation

KRT18

Uniprot NO.

Q5BJY9

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma,tissue homogenates

Detection Range

0.94 ng/mL-60 ng/mL

Sensitivity

0.23 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat KRT18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	99
	Range %	94-103
1:4	Average %	101
	Range %	98-105
1:8	Average %	98
	Range %	93-104

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.606

2.657

2.632

2.469

1.798

1.774

1.786

1.623

1.152

1.198

1.175

1.012

7.5

0.713

0.739

0.726

0.563

3.75

0.446

0.468

0.457

0.294

1.87

0.334

0.348

0.341

0.178

0.94

0.265

0.267

0.266

0.103

0.161

0.165

0.163

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rat KRT18 ELISA Kit was designed for the quantitative measurement of Rat KRT18 protein in serum, plasma,tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.94 ng/mL-60 ng/mL and the sensitivity is 0.23 ng/mL.

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■ Q&A

1. Which is this standard in this kit, natural material or recombinant? If recombinant, please inform me on the expression method (E.coli etc.).
2. Does this kit include polyclonal antibody (except for microplate binding protein)? If so, please tell me the derivation and purification method.
3. Is the standard or antibody, including any additive agent (antiseptic agent or preserving agent)? If so, please let me know the composition and concentration.
4. Does this product include a Stop solution? If so, please tell me the composition and concentration for the stop solution.
5. Please let me know whether this kit include any hazardous substances (azide, thimerosal, 2-ME, etc). If so, please inform me of the composition and concentration.

Thanks for your inquiry!
Pls check our answer:
1, Standard of CSB-EQ027319RA is NS0-derived recombinant protein
2, Coated anti: Mouse monoclonal antibody(precoated in the plate); Detection anti: Goat polyclonal(affinity purification)
3, Stop solution: 4N sulfuric acid
4, There is no hazardous substances (azide, thimerosal, 2-ME, etc) included in the kit.
5, Preservative of this kit is proclin 300(1:3000).

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells.

Gene References into Functions

Resutls show that the appearance caspase-cleaved CK18 in plasma was found to reflect formation of the caspase-cleaved epitope in PMID:19407366

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Subcellular Location

Nucleus matrix. Nucleus, nucleolus. Cytoplasm.

Protein Families

Intermediate filament family

Tissue Specificity

Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine

Database Links

UNIGENE: Rn.103924

KEGG: rno:294853

STRING: 10116.ENSRNOP00000067234

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