Rat cyclooxygenase-2,COX-2 ELISA Kit

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Name: Rat cyclooxygenase-2,COX-2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E13399r

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Code: CSB-E13399r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 1.56 ng/mL-100 ng/mL

Sensitivity: 0.39 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit

Abbreviation

PTGS2

Uniprot NO.

P35355

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-106
1:2	Average %	95
	Range %	91-99
1:4	Average %	92
	Range %	88-96
1:8	Average %	85
	Range %	80-89

Recovery

The recovery of rat COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.386	2.285	2.336	2.191
50	1.628	1.527	1.578	1.433
25	0.937	0.916	0.927	0.782
12.5	0.564	0.575	0.570	0.425
6.25	0.365	0.376	0.371	0.226
3.12	0.240	0.251	0.246	0.101
1.56	0.195	0.191	0.193	0.048
0	0.145	0.144	0.145

Troubleshooting and FAQs

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ELISA Data Analysis

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Rat cyclooxygenase-2,COX-2 ELISA Kit

Description

The rat COX-2 ELISA Kit quantitates rat COX-2 levels in serum, plasma, and tissue homogenates. COX-2 (PTGS2) is the key rate-limiting enzyme needed for the synthesis of prostanoids, including PGE2, PGD2, PGF2a, PGI2, and TAX2, from an essential fatty acid arachidonic acid (AA). It is constitutively expressed in certain cell groups of the brain and inducibly expressed in response to inflammatory reactions. In the central nervous system (CNS), COX-2 affects memory, sensory integration, and autonomic modulation. COX-2 overexpression is mainly linked to inflammation, apoptosis inhibition, uncontrolled cell proliferation, growth, metastasis, neovascularization, and angiogenesis finally resulting in cancer. COX-2 is often expressed in many malignant tumors and is associated with occurrence, progression, and prognosis.

This kit employs the sandwich-ELISA mechanism in conjugation with COX-2 antibody-COX-2 antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of COX-2 in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.

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■ Q&A

How can I convert pyggrams per decilitre to picograms per milligram, because your kits are in deciliters and concentrates
picogeram/dl---picogeram/mg
this kits according to picogerm/dl, i want according to picogeram /mg

Thanks for your inquiry. For the kit indicated,convertions are not suggested.We recommand you caculate according to the unit stated in the manual.
Pls let me know if you have any further questions. Thank you.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia.

Gene References into Functions

Increased expression of COX-2 is associated with aortic endothelial dysfunction. PMID:30292829
findings indicated that BMP9 is involved in the phosphate-induced calcification in VSMCs and COX-2 partly mediates the BMP9-induced calcification in VSMCs through activating Wnt/beta-catenin pathway. PMID:29073723
the cortical COX2 pathway was activated in CUMS rats. Inhibition of COX2 activity/expression can obviously improve depressive behaviours in CUMS rats. Upregulating COX2 expression can increase the susceptibility of rats to CUMS. PMID:28352129
The present study proposed that COX-2-mediated inflammation is important as a candidate target for hippocampal impairment in the hypothyroidism. PMID:29436670
COX1 and 2 gene expression and localization in the injured brain regulating prostaglandin synthase. PMID:28933223
In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. PMID:28508833
Microglial TNFalpha induces cyclooxygenase 2 (COX2) and PGI2 synthase expression in spinal endothelial cells during neuropathic pain. These findings further suggest that the glia-endothelial cell interaction of the neurovascular unit via transient TNFalpha is involved in the generation of neuropathic pain. PMID:28451639
The authors measured brain oxygenation in localized areas from freely-moving rodents and discovered a severe hypoxic event (pO2 < 10 mmHg) after the termination of seizures. This event lasted over an hour, is mediated by hypoperfusion, generalizes to people with epilepsy, and is attenuated by inhibiting cyclooxygenase-2 or L-type calcium channels. PMID:27874832
C2-ceramide enhances phenylephrine-induced vascular smooth muscle constriction by mediation of the ER stress/COX-2/PGE2 pathway. PMID:28797762
Results point to an excess of reactive oxygen species mainly from NAD(P)H oxidase after aluminum exposure and increased vascular prostanoids from COX-2 acting in concert to decrease NO bioavailability, thus inducing vascular dysfunction and increasing blood pressure. PMID:28826906
Hepatic COX2 expression and PGI2 production are enhanced in cirrhotic rats, but the correlation with Hepatic encephalopathy (HE) is not remarkable. PMID:27580512
a stiffness-dependent YAP/TAZ-mediated positive feedback loop that drives remodeling phenotypes in pulmonary artery smooth muscle cells via reduced COX-2 and prostaglandin activity. PMID:28642262
the disrupted pattern of hippocampal neurogenesis induced by MNU is different between developmental and postpubertal exposure and is dependant on COX2 regulation. PMID:28688903
the long-lasting oligemia following cortical spreading depression consists of at least two distinct temporal phases, mediated by preferential actions of COX-1- and COX-2-derived prostanoids: an initial phase mediated by COX-1 that involves TXA2 followed by a later phase, mediated by COX-2 and PGF2alpha. PMID:27178425
These data suggest that adaptive changes induced in the myocardium by CIH may include activation of cPLA2alpha and COX-2 via beta2-AR/Gi-mediated stimulation of the ERK/p38 pathway. PMID:27686454
COX-2 protein expression was upregulated in lung tissue in response to skeletal muscle ischemia reperfusion PMID:26724476
It was concluded that intestinal damage during acute pancreatitis is exacerbated in elderly rats compared with young rats and that COX-2 inhibition could be a potential therapeutic target to offer tailored treatment for acute pancreatitis in the elderly. PMID:26610611
TNFalpha-RelB/p52 pathway may be involved in the early stages of renal damage, in part by stimulating COX-2 and inflammatory responses PMID:26824492
COX1/2 inhibition alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury PMID:26612417
Low-salt diet modulates mesenteric vascular responses via increased NO bioavailability suggested by increased iNOS protein expression and vasoconstrictor prostanoid production via COX-2. PMID:26685759
These results suggest the existence of a causal link between Cox-2 and p53, which may represent a toxic mechanism of electrophilic lipid peroxidation products. PMID:25506925
Src modulates the 4-Hydroxynonenal-enhanced activation of AP-1 and the expression of COX-2 PMID:26466383
IL-1beta reduces tonic contraction of mesenteric lymphatic muscle cells, with the involvement of COX-2 and prostaglandin E2 PMID:25989136
Data show that mesenchymal stem cells (MSCs) administration alleviated airway inflammation and emphysema through the down-regulation of cyclooxygenase-2 (COX-2) via p38 and ERK MAPK pathways. PMID:25736434
increased COX-2-dependent vasoconstriction contributes to renal endothelial dysfunction through enhanced reactive oxygen species generation in obesity. PMID:25841778
The aim of this study was to determine the role of COX-2 in the regulation of blood pressure and to define the mechanisms in two kidney one-clip hypertensive (2K1C) rats PMID:25846103
Treatment with probiotics has the potential of providing protection against colon cancer by down-regulating the COX-2 expression as one of the protective mechanisms. PMID:25811420
TNF-alpha-induced COX-2 and PGE2 stimulate Wnt signaling and activate Wnt target genes PMID:26123748
Increased COX2 expression after l-DOPA long-term treatment in Parkinsonian-like rats could contribute to the development of dyskinesia. PMID:26009769
Concomitant use of alpha-lipoic acid and cyclosporine significantly increased nitric oxide production, cyclooxygenase-2 and miR-210 gene expression in rat colon. PMID:26276312
E2-BSA induced the COX-2 expression and consequent PGE2 and PGF2alpha release in rat OECs. These effects are mediated through GPR30-derived EGFR transactivation and PI-3K/Akt cascade leading to NF-kappaB activation PMID:26241029
Low-level laser therapy decreases Achilles tendon inflammatory process mediated by Il1b and COX-2. PMID:25070591
COX-2 oxidative metabolism is a key regulator of the anandamide-mediated decidual remodeling in pregnancy. PMID:26335727
Our results demonstrated that ventricular hypertrophy abrogated isoflurane-induced delayed cardioprotection by alteration of iNOS/COX-2 pathway PMID:25400168
Hypomethylation of PTGS2, and hypermethylation of APC2 may be causally linked to the etiology of oral cancer in a rat model. PMID:25635769
Clathrin-dependent internalization of the angiotensin II ATA receptor links receptor internalization to COX-2 protein expression in rat aortic vascular smooth muscle cells PMID:25542758
Report design of benzimidazole analogs endowed with oxadiazole as selective COX-2 inhibitors. PMID:25303727
Cadmum induced release of reactive oxygen species derived from NADPH oxidase may be due to the increased local release of angiotensin II and the release of vasoconstrictor prostanoids derived from the COX-2 pathway. PMID:23973752
Data suggest that cyclooxygenase-2 (COX2) and NADPHox are potential therapeutic targets to decrease testosterone-driven cardiovascular risk. PMID:25700020
data propose that an increase in COX-2 expression in ventral CA1 following trauma is likely due to its attenuated degradation. PMID:25058273
Data (including data from studies in transgenic rats) suggest that attenuation of circadian clock system, especially its core component Rev-erb-alpha, contributes to induction of Ptgs2 in endometrial stromal cells during decidualization/placentation. PMID:25648833
Nitric oxide synthase (NOS) and cyclooxygenase (COX) isoform expression patterns were determined in femoral artery of obese Zucker rats. PMID:25216050
Reflux of duodenal contents induces COX2 expression and increases prostaglandin synthesis in dysplastic and cancer tissues PMID:24914375
ischemia reperfusion injury significantly increased the COX2 expression, PGI2 and TXA2 levels, and the PGI2/TXA2 ratio in rat hippocampus in a time-dependent manner PMID:25388440
The present study showed that the group receiving Vitamin E supplementation at 3x recommended daily intake displayed reduced COX2 expression. PMID:25123248
during the early phase of ANG II-dependent hypertension, the increased (P)RR and COX-2 expression in the renal medulla may contribute to attenuate the vasoconstrictor effects of ANG II on renal hemodynamics PMID:25143455
The data suggest that local hypertonicity in the medullary thick ascending limb is associated with an increase in COX-2 expression concomitant with elevated EP3 receptor expression, which limits COX-2 activity in this segment of the nephron. PMID:25080527
Elevated COX-2 expression during hypoxia where angiogenesis occurs and re-oxygenation, when micro-vessels regress, identifies this protein as a vascular remodeling molecule crucial for angioplasticity. PMID:24796880
Fibroblasts activated by hepatoma cell supernatant express COX-2 and HGF at higher levels. PMID:24815169
Suggest lead-associated inflammatory neurotoxicity occurs via activation of pro-inflammatory NFAT3/COX-2 axis. PMID:25193092

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Subcellular Location

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus inner membrane; Peripheral membrane protein. Nucleus outer membrane; Peripheral membrane protein.

Protein Families

Prostaglandin G/H synthase family

Tissue Specificity

Expressed throughout the forebrain in discrete populations of neurons and is enriched in the cortex and hippocampus.

Database Links

UNIGENE: Rn.217585

KEGG: rno:29527

STRING: 10116.ENSRNOP00000003567

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