Rat cartilage oligomeric matrix protein,COMP ELISA Kit

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Rat cartilage oligomeric matrix protein,COMP ELISA Kit

Name: Rat cartilage oligomeric matrix protein,COMP ELISA Kit
Brand: CUSABIO
SKU: CSB-E13833r

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Code: CSB-E13833r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 78 pg/ml - 5000 pg/ml

Sensitivity: 19.5 pg/ml

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

CompCartilage oligomeric matrix protein ELISA kit; COMP ELISA kit

Abbreviation

COMP

Uniprot NO.

P35444

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

78 pg/ml - 5000 pg/ml

Sensitivity

19.5 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat COMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-107
1:2	Average %	97
	Range %	91-105
1:4	Average %	98
	Range %	92-104
1:8	Average %	100
	Range %	95-105

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

5000

2.639

2.699

2.669

2.501

2500

2.151

2.163

2.157

1.989

1250

1.505

1.542

1.524

1.356

625

1.062

1.098

1.080

0.912

312

0.637

0.689

0.663

0.495

156

0.392

0.399

0.396

0.228

0.332

0.347

0.340

0.172

0.168

0.167

0.168

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The product CSB-E13833r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat cartilage oligomeric matrix protein (COMP) in biological samples, including serum, plasma, or tissue homogenates. This kit has undergone rigorous quality tests in multiple parameters, including specificity, sensitivity, precision, and lot-to-lot consistency.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. COMP in the samples or standards are sandwiched between pre-coated COMP antibody and Biotin-conjugated COMP antibody. The COMP/pre-coated COMP antibody/Biotin-conjugated COMP antibody complex is labeled by HRP and then occurs a chromogenic reaction after the addition of the TMB solution. The color intensity is positively related to the COMP in the samples.

COMP, also known as thrombospondin-5 (TSP-5), is a matricellular protein essential for maintaining vascular homeostasis. It also plays a crucial role in cartilage and bone metabolism and is involved in arthritis. COMP preserves the contractile phenotype of vascular smooth muscle cells and prevents the formation of vascular neointima and vascular calcification after an injury. Model mice lacking COMP developed spontaneous dilated cardiomyopathy and heart failure. Evidence revealed that COMP is an endogenous thrombin inhibitor and negatively modulates hemostasis and thrombosis.

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Salmon calcitonin exerts better preventive effects than celecoxib on lumbar facet joint degeneration and long-term tactile allodynia in rats Gou Y, et al,/,2019
A putative Chondroprotective role for IL-1B and MPO in herbal treatment of experimental osteoarthritis Aborehab NM.et al,/,2017
Salmon Calcitonin Attenuates Degenerative Changes in Cartilage and Subchondral Bone in Lumbar Facet Joint in an Experimental Rat Model Yu Gou.et al,/,2018
Particulate matter exposure aggravates osteoarthritis severity Peng K T, et al,/,2019
S-Allylmercaptocysteine Targets Nrf2 in Osteoarthritis Treatment Through NOX4/NF-κB Pathway G Yang,/,2020
Microencapsulated Recombinant Human Epidermal Growth Factor Ameliorates Osteoarthritis in a Murine Model SC Lin,/,2021
Exposure to ambient air pollution and osteoarthritis; an animal study A Fitriyah,/,2022
Achyranthoside D attenuates chondrocyte loss and inflammation in osteoarthritis via targeted regulation of Wnt3a W Xie,/,2023
Therapeutic effects of combining curcumin and swimming in osteoarthritis using a rat model MM Saber,/,2023
Parathyroid hormone (1–34) retards the lumbar facet joint degeneration and activates Wnt/β-catenin signaling pathway in ovariectomized rats Y Gou,/,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.

Gene References into Functions

COMP could normally have a protective role against PASMC phenotype switching PMID:28860005
these findings revealed the essential role of COMP in retarding the development of vascular aging and vascular smooth muscle cell senescence. PMID:27498005
COMP-Ang1-stimulated proliferation, differentiation, and migration of progenitor cells may involve the Tie-2-mediated activation of p38 MAPK and PI3K/Akt pathways PMID:25446117
results suggest the potential importance of COMP-Ang1 as a new therapy for impaired bone formation that is associated with diabetes and advanced age PMID:23850469
The presence of cleaved apoptosis inducing factor (tAIF) and the absence of activated caspases indicated that retention of D469del-COMP triggers cell death in chondrocytes by necroptosis, a caspase-independent programmed necrosis. PMID:22154936
Cartilage oligomeric matrix protein is a novel inhibitor of vascular smooth muscle calcification. PMID:21350216
Overexpression of COMP markedly suppressed PDGF-BB-elicited VSMC dedifferentiation. Alpha(7)beta(1) integrin is a potential receptor for COMP. PMID:20019333
Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin. PMID:12225811
The X-ray structure of the COMP coiled coil domain in complex with vitamin D(3) at 1.7 A resolution is reported. COMP exerts a distinct mechanism of interaction mainly defined by the pattern of hydrophobic core residues. PMID:12426368
COMP has a role in development of the extracellular matrix and in a cell culture model for pseudoachondroplasia PMID:15579310
COMP secretion is affected by the signal peptide PMID:15749701
Expression of COMP in both anulus fibrosus(AF) and nucleus pulposus(NP) of intervertebral disc(IVD). COMP is component of extracellular matrix of AF and NP, with lamellar distribution in AF. May play role in normal structure of IVD. PMID:16778685
ultrastructural distribution of COMP & TSP-4 & collagen fibril thickness in achilles tendon related to exercise; COMP immunolabeling decreased with longer running distance & was lower in pericellular & interterritorial compartments of high training rats PMID:17882701
To identify residues critical for the structure, stability, oligomerization and binding to vitamin D as well as two small molecules, all-trans-retinol and curcumin, an alanine-scanning mutagenesis study of cartilage oligomeric matrix protein is performed. PMID:19681593