Rat apolipoprotein H (Apo-H) ELISA Kit

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Name: Rat apolipoprotein H (Apo-H) ELISA Kit
Brand: CUSABIO
SKU: CSB-E10073r

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Code: CSB-E10073r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 28 ng/mL-1800 ng/mL

Sensitivity: 7 ng/mL

Trial Size:

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Target Background

Target Name

apolipoprotein H (beta-2-glycoprotein I)

Alternative Names

ApohBeta-2-glycoprotein 1 ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit

Abbreviation

APOH

Uniprot NO.

P26644

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

28 ng/mL-1800 ng/mL

Sensitivity

7 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	102
	Range %	97-107
1:800	Average %	91
	Range %	85-97
1:1600	Average %	97
	Range %	91-103

Recovery

The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	107	101-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1800	2.655	2.532	2.594	2.464
900	2.164	2.117	2.141	2.011
450	1.597	1.544	1.571	1.441
225	0.845	0.828	0.837	0.707
112	0.501	0.511	0.506	0.376
56	0.307	0.300	0.304	0.174
28	0.234	0.222	0.228	0.098
0	0.131	0.129	0.130

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat apolipoprotein H (Apo-H) ELISA Kit is the reliable and accurate solution for detecting and quantifying Beta-2-glycoprotein 1, also known as Apolipoprotein H (Apo-H) or Beta-2-glycoprotein I (B2GPI), in rat serum, plasma, and tissue homogenates.

With its detection range of 28 ng/mL-1800 ng/mL and a sensitivity of 7 ng/mL, this ELISA kit allows for precise measurements of Apo-H levels in a variety of sample types. The assay time is 1-5 hours, and the sample volume required is only 50-100 µL, making it a convenient and efficient choice for researchers in the cardiovascular field.

The Rat apolipoprotein H (Apo-H) ELISA Kit follows a sandwich assay principle, where the sample is captured by an immobilized antibody specific to rat Apo-H and then detected by a biotinylated antibody. The amount of detected signal is proportional to the concentration of Apo-H in the sample, which is measured at a detection wavelength of 450 nm. The high sensitivity, wide detection range, and easy-to-use protocol make this kit an ideal choice for researchers in the area.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Subcellular Location

Secreted.

Tissue Specificity

Expressed by the liver and secreted in plasma.

Database Links

UNIGENE: Rn.1824

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