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Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

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Code: CSB-E07352r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.312 ng/mL-20 ng/mL

Sensitivity: 0.078 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Product Details

Target Name

c-fos induced growth factor (vascular endothelial growth factor D)

Alternative Names

Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit

Abbreviation

Vegfd

Uniprot NO.

O35251

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-92
1:2	Average %	101
	Range %	97-105
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-95
EDTA plasma (n=4)	98	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.932	1.837	1.885	1.738
10	1.456	1.566	1.511	1.364
5	1.088	1.042	1.065	0.918
2.5	0.713	0.707	0.710	0.563
1.25	0.492	0.481	0.487	0.340
0.625	0.358	0.342	0.350	0.203
0.312	0.272	0.278	0.275	0.128
0	0.148	0.146	0.147

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is a powerful tool for researchers studying the cardiovascular system in rats. VEGF-D is a protein known for its role in promoting the growth and survival of blood vessels, making it an important target for cardiovascular research.

Our ELISA kit utilizes a quantitative sandwich assay principle to measure the levels of VEGF-D in serum, plasma, and tissue homogenates with a detection range of 0.312 ng/mL to 20 ng/mL and a sensitivity of 0.078 ng/mL. With an assay time of only 1-5 hours and a sample volume of 50-100ul, this kit is both efficient and convenient for researchers.

Whether you are investigating the mechanisms of angiogenesis and lymphatic regeneration or studying the effects of cardiovascular disease on the vasculature, this Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is the perfect tool for your research needs.

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Citations

Spatially Heterogeneous Microgel-Annealed Fibrin Hydrogel Promotes Coordinated Vasculogenesis and Angiogenesis After Myocardial Infarction Y Huangfu, Y Zhang, Y Zhang, R Gao, S Wang,/,/

Target Background

Function(From Uniprot)

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor.

Gene References into Functions

Expression of VEGF-D and its receptor, VEGFR-3, are enhanced in various cell types in the infarcted heart. PMID:22818386
Adenovirus encoding vascular endothelial growth factor-D induces tissue-specific vascular patterns in vivo. PMID:12036873
Overexpression of VEGF-D is associated with Gliosarcomas PMID:16533777
VEGF-D expression may have a role in interleukin (IL)-6 expression and transcription factor AP-1 activation in pancreatic acinar cells. PMID:17404025