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Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

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Code: CSB-E07380r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.625 ng/mL-40 ng/mL

Sensitivity: 0.156 ng/mL

Trial Size:

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Target Background

Product Details

Target Name

Tumor necrosis factor soluble receptor Ⅱ,TNFsR-

Alternative Names

Tnfrsf1b ELISA Kit; Tnfr2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; TNF-R2 ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; TNF-RII ELISA Kit; TNFR-II ELISA Kit; p75 ELISA Kit; p80 TNF-alpha receptor ELISA Kit; CD antigen CD120b ELISA Kit

Abbreviation

TNFsR2

Uniprot NO.

Q80WY6

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	87-98
1:8	Average %	92
	Range %	85-95

Recovery

The recovery of rat TNFsR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-96
EDTA plasma (n=4)	98	95-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.827	2.899	2.863	2.732
20	2.222	2.323	2.273	2.142
10	1.373	1.412	1.393	1.262
5	0.816	0.866	0.841	0.710
2.5	0.531	0.545	0.538	0.407
1.25	0.315	0.323	0.319	0.188
0.625	0.226	0.241	0.234	0.103
0	0.126	0.135	0.131

Troubleshooting and FAQs

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Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

Description

This Rat TNFsR2 ELISA Kit was designed for the quantitative measurement of Rat TNFsR2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function(From Uniprot)

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2.

Gene References into Functions

results suggested that PTE may promote BMSC proliferation and osteogenic differentiation via a mechanism associated with the regulation of Let-7f-5p and the TNFR2/PI3K/AKT signaling pathway. PMID:29975930
Curcumin decreased TNFR2 and increased TRAF2 to decrease the apoptotic pathway in ureteral obstruction rats. PMID:26732833
The IL-17RD.TNFR2 complex in activation of NF-kappaB may explain the role of TNFR2 in inflammatory diseases including nephritis. PMID:25378394
the microglial TNF-alpha p55 pathway played a more important role than the TNF-alpha p75 pathway in the pathogenesis of peripheral nerve injury pain. PMID:22652595
study of TNF-alpha and TnfrR2 protein synthesis and expression patterns in sciatic nerve of controls and rats under systemic challenge with lipopolysaccharide. PMID:17873366
In human biopsies and in rat kidney allografts on day 6 with acute allograft rejection, significantly elevated expression of TNFR2 was observed. PMID:19298452