Rat Transforming Growth factor β2,TGF-β2 ELISA kit

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Rat Transforming Growth factor β2,TGF-β2 ELISA kit

Name: Rat Transforming Growth factor β2,TGF-β2 ELISA kit
Brand: CUSABIO
SKU: CSB-E12111r

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Code: CSB-E12111r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Tgfb2 ELISA Kit; Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit

Abbreviation

TGFB2

Uniprot NO.

Q07257

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TGF-β2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	95
	Range %	91-99
1:4	Average %	87
	Range %	82-90
1:8	Average %	95
	Range %	91-99

Recovery

The recovery of rat TGF-β2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	81-85
EDTA plasma (n=4)	96	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.869	2.897	2.883	2.691
500	2.231	2.267	2.249	2.057
250	1.544	1.598	1.571	1.379
125	0.969	0.987	0.978	0.786
62.5	0.638	0.609	0.624	0.432
31.2	0.452	0.472	0.462	0.270
15.6	0.307	0.321	0.314	0.122
0	0.191	0.193	0.192

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat Transforming Growth factor β2 (TGF-β2) ELISA kit is a high-quality tool for quantitative measurement of TGF-β2 levels in rat serum, plasma, and tissue homogenates.

The kit is designed to detect Transforming growth factor beta-2 proprotein, encoded by the Tgfb2 gene in Rattus norvegicus (Rat), which plays a key role in signal transduction pathways,cell growth, cell differentiation, apoptosis and cellular homeostasis.

With a wide detection range of 15.6 pg/mL-1000 pg/mL and a sensitivity of 3.9 pg/mL, this sandwich ELISA kit ensures accurate and precise measurements of TGF-β2 levels, even at low concentrations. The assay time is quick, ranging from 1-5 hours, with a sample volume of only 50-100ul.

The Rat Transforming Growth factor β2 (TGF-β2) ELISA kit is ideal for researchers studying signal transduction in the context of various diseases and conditions. The kit is simple to use, and the results are easily readable at a detection wavelength of 450 nm.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.

Gene References into Functions

VEGF and TGFB2 are upregulated in femoral bone during fracture healing following implantation with coated titanium screws. PMID:28361415
Diminished collagen synthesis in folliculostellate cell-deficient cell aggregates was partially recovered by TGFbeta2. TGFbeta2-mediated folliculostellate cell-pericyte interaction appears to be essential for collagen synthesis in anterior pituitary. PMID:26957638
miR-378a expression is associated with its methylation status in TGF-beta1-treated cells, and epigenetically-regulated miR-378a inhibits TGF-beta1-induced hepatic stellate cells activation, at least in part, via TGF-beta2. PMID:27855367
Hepatic TGFB2 gene expression was up-regulated in liver of bile duct ligation model of liver fibrosis. PMID:27049496
These study results suggest that HR controls Tgf-b2 expression via regulation of miR-31, thus causing abnormal hair cycle in Hr(Hp)/Hr(Hp) mice. PMID:25639665
Data suggest that Muller cells release TGF-beta2, inhibiting the proliferation of retinal endothelial cells via activation of Smad2/Smad3 and attenuation of ERK signaling PMID:24838936
level of TGFbeta2 in rat colonic neoplasms PMID:12209600
Tgfb2 exhibited dynamic changes in expression in experimental glomerulonephritis. PMID:12545247
colocalizes with the trans-Golgi network marker TGN38 and a marker molecule for secretory granules, chromogranin B in PC12 cells and hippocampal neurons; a substantial portion of TGF-beta2 is secreted by the regulated secretory pathway in these cells PMID:12595240
TGF-beta 2 may play a physiological role in germ cell proliferation/differentiation and Sertoli cell function. PMID:12646048
role in cranial suture fusion PMID:12733949
Tgfb2 exrpession increased in infarcted myocardium after propranolol treatment. PMID:14575314
Analysis of Tgf-beta2 expression patterns in acute and progressive glomerulonephritis models. PMID:15122060
TGF-beta2 mRNA and protein expressions in the cerebral cortex of the H-Tx rat, a model of congenital hydrocephalus. PMID:15375625
TGF-beta2 plays important role in pancreatic morphogenesis and administration of excess all-trans retinoic acid before neurulation inhibit TGF-beta2 expression disrupted pancreatic morphogenesis particularly Langerhans islets. PMID:16479313
differential processing of TGF-beta1 and -beta 2 may be part of the mechanism that generates isoform-specific functions of the TGF-betas PMID:17516499
expression and signaling of TGFbeta2 and distribution of extracellular matrix proteins: laminin, fibronectin, collagens in the parotid gland of control and diabetic rats. PMID:17574405
novel role for TGF-beta2 in protection from unloading-induced apoptosis in vivo PMID:18378961
TGF-beta1 and TGF-beta 2 mRNA, but not TGF-beta3, is increased at 4 and 12 weeks in experimental diabetic peripheral neuropathy. PMID:18406405
immunolocalization of transforming growth factor beta (TGF-beta2) in rat thymic stromal cells and thymocytes: TGF-beta2 may play an important role in the organization or development of thymocytes in the late stages of thymopoiesis PMID:18554691
These results show that elevated expression of TSP-1 and TGF-beta can be detected in the injured segment of the spinal cord 12 and 24 h after injury. Thus, TSP-1 and TGF-beta may have a role in SCI. PMID:19342245
The renoprotective effect of AM might be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-beta1 expression. PMID:19434765
TGF-beta2 is an essential neuroprotective factor released by microglia in its culture medium that, to be fully effective, requires the concomitant presence of other factor(s) of low molecular weight. PMID:19457129
TGF-beta2 and TGF-beta3 induced a dose-dependant increase of apoptosis in cultured stromal cells. PMID:19656380

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Subcellular Location

[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.

Protein Families

TGF-beta family

Tissue Specificity

[Isoform TGF-beta2B]: Expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine.

Database Links

UNIGENE: Rn.24539

KEGG: rno:81809

STRING: 10116.ENSRNOP00000003313

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