Rat Thyroglobulin,TG ELISA Kit

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Code: CSB-E08240r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue Homogenates

Detect Range: 10 ng/mL-160 ng/mL

Sensitivity: 10 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details
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Target Background

Product Details

Target Name

thyroglobulin

Alternative Names

TgThyroglobulin ELISA kit; Tg ELISA kit

Abbreviation

Uniprot NO.

P06882

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue Homogenates

Detection Range

10 ng/mL-160 ng/mL

Sensitivity

10 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	92
	Range %	86-99
1:4	Average %	95
	Range %	87-105
1:8	Average %	90
	Range %	83-102

Recovery

The recovery of rat TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	88-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
10	2.553	2.568	2.561
20	1.748	1.763	1.756
40	0.885	0.875	0.880
80	0.515	0.511	0.513
160	0.285	0.287	0.286

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat Thyroglobulin (TG) ELISA Kit is a perfect solution for researchers studying metabolism，thyroid-related diseases. This quantitative assay kit allows for the detection of Thyroglobulin (Tg) in serum, plasma, and tissue homogenates of Rattus norvegicus (rat) samples.

With a detection range of 10 ng/mL to 160 ng/mL and a sensitivity of 10 ng/mL, this ELISA kit provides accurate and reliable results for your research needs. The assay time is quick, ranging from 1-5 hours, and the sample volume required is only 50-100 ul.

The detection wavelength for this kit is 450 nm, providing high sensitivity and specificity for the measurement of Tg in rat samples. The assay principle is based on the competitive method, providing high specificity and accuracy for the detection of this protein.

This Rat Thyroglobulin (TG) ELISA Kit has been cited in over 5 peer-reviewed publications, demonstrating the reliability and accuracy of this kit in the scientific community.

Customer Reviews and Q&A

■ Customer Reviews

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Citations

A comparison of the thyroid disruption induced by decabrominated diphenyl ethers (BDE-209) and decabromodiphenyl ethane (DBDPE) in rats Wang Y, et al,/,2019
Altered thyroid hormone production induced by long-term exposure to low doses of the endocrine disruptor dichlorodiphenyltrichloroethane Yaglova N V.et al,/,2015
Mechanisms of Disruptive Action of Dichlorodiphenyltrichloroethane (DDT) on the Function of Thyroid Follicular Epitheliocytes. Yaglova NV. et al,/,2015
Rutin protects against aging-related metabolic dysfunction Li T. et al,/,2016
ER stress contributes to high fat diet induced decrease of thyroglobulin and hypothyroidism Zhang X, et al,/,2019
The Effects of Alpha Lipoic Acid on Oxidative Stress, Lipid Profile, and Blood Glucose Levels in Type 2 Diabetic Male Sprague Dawley Rats BU Khan,/,2024
The ameliorative role of germinated fenugreek and barley grains on the structure and function of offspring's tibialis muscle maternally fed on high fat diet FA Hassan Megahed,/,2024

Target Background

Function(From Uniprot)

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.

Subcellular Location

Secreted.

Protein Families

Type-B carboxylesterase/lipase family

Tissue Specificity

Specifically expressed in the thyroid gland.

Database Links

UNIGENE: Rn.10429

STRING: 10116.ENSRNOP00000009241

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