Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

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Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

Name: Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit
Brand: CUSABIO
SKU: CSB-E08729r

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Code: CSB-E08729r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 0.78 pg/mL-50 pg/mL

Sensitivity: 0.195 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Target Name

Stromal cell derived factor 1a,SDF-1a

Abbreviation

SDF1a/CXCL12

Uniprot NO.

Q9QZD1

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.78 pg/mL-50 pg/mL

Sensitivity

0.195 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	83-97
1:200	Average %	97
	Range %	91-103
1:400	Average %	84
	Range %	80-88
1:800	Average %	89
	Range %	81-95

Recovery

The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	97	94-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50	2.830	2.775	2.803	2.666
25	2.326	2.453	2.390	2.253
12.5	1.600	1.632	1.616	1.479
6.25	0.937	0.894	0.916	0.779
3.12	0.528	0.532	0.530	0.393
1.56	0.321	0.335	0.328	0.191
0.78	0.157	0.148	0.153	0.016
0	0.140	0.133	0.137

Troubleshooting and FAQs

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ELISA Data Analysis

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Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

Description

The Rat Stromal cell-derived factor 1a (SDF-1a) ELISA Kit is a reliable and efficient solution for researchers in the field of immunology. This ELISA kit enables the quantitative measurement of SDF-1a in a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates from Rattus norvegicus (Rat).

With a detection range of 0.78 pg/mL to 50 pg/mL and a sensitivity of 0.195 pg/mL, this ELISA kit delivers accurate and precise results, making it a valuable tool for researchers working on SDF-1a in rats. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

This ELISA kit works on a sandwich assay principle, where the SDF-1a antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

The Rat SDF-1a ELISA Kit has been cited in more than 4 publications, highlighting its reliability and utility in research. It is an excellent addition to any immunology research laboratory, providing accurate and precise measurement of SDF-1a in rat samples.