Rat Runt-related transcription factor 2(RUNX2) ELISA kit

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Rat Runt-related transcription factor 2(RUNX2) ELISA kit

Name: Rat Runt-related transcription factor 2(RUNX2) ELISA kit
Brand: CUSABIO
SKU: CSB-EL020594RA

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Code: CSB-EL020594RA

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell lysates

Detect Range: 3.12 ng/mL-200 ng/mL

Sensitivity: 0.78 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

Runx2 ELISA Kit; Cbfa1 ELISA Kit; Osf2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; CBF-alpha-1 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OSF-2 ELISA Kit; Fragments ELISA Kit

Abbreviation

RUNX2

Uniprot NO.

Q9Z2J9

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates, cell lysates

Detection Range

3.12 ng/mL-200 ng/mL

Sensitivity

0.78 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat RUNX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-103
1:2	Average %	102
	Range %	94-107
1:4	Average %	88
	Range %	83-91
1:8	Average %	94
	Range %	84-98

Recovery

The recovery of rat RUNX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	93-107
EDTA plasma (n=4)	98	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.551	2.452	2.502	2.322
100	2.126	1.996	2.061	1.881
50	1.359	1.341	1.350	1.170
25	0.793	0.818	0.806	0.626
12.5	0.474	0.481	0.478	0.298
6.25	0.395	0.403	0.399	0.219
3.12	0.266	0.257	0.262	0.082
0	0.182	0.178	0.180

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Rat Runt-related transcription factor 2(RUNX2) ELISA kit

Description

The Rat Runt-related transcription factor 2 (RUNX2) ELISA kit is a powerful tool for the quantitative measurement of RUNX2 protein levels in serum, plasma, tissue homogenates, and cell lysates of Rattus norvegicus (Rat) samples.

RUNX2, also known as CBFA1, is a transcription factor that plays a critical role in bone development and remodeling，signal pathway. Our ELISA kit is specifically designed for the measurement of RUNX2 protein levels, making it an ideal tool for studying the molecular mechanisms underlying bone formation and skeletal disorders in rats.

With a detection range of 3.12 ng/mL to 200 ng/mL and a sensitivity of 0.78 ng/mL, our RUNX2 ELISA kit offers exceptional accuracy and precision in measuring protein levels. The assay time is only 1-5 hours, and the sample volume required is just 50-100ul. The detection wavelength is 450 nm, and the assay principle is quantitative, utilizing a sandwich method for precise measurements.

Our RUNX2 ELISA kit is suitable for researchers studying epigenetics and nuclear signaling in the context of bone development and disease. It is user-friendly and easy to use, providing reliable results for the research applications.

■ Customer Reviews

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Tofu-Incorporated Hydrogels for Potential Bone Regeneration K Huang,/,2020
Improvement Effect and Regulation Mechanism of Oyster Peptide on Dexamethasone-Induced Osteoporotic Rats W Yang, W Ma, X Qin, W Cao, H Lin,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).

Subcellular Location

Nucleus.

Database Links

UNIGENE: Rn.83672

STRING: 10116.ENSRNOP00000057733

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