Rat Platelet Factor 4,PF-4 ELISA Kit

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Name: Rat Platelet Factor 4,PF-4 ELISA Kit
Brand: CUSABIO
SKU: CSB-E07885r

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Code: CSB-E07885r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.156 μg/mL-10 μg/mL

Sensitivity: 0.039 μg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

platelet factor 4

Alternative Names

Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit

Abbreviation

PF4

Uniprot NO.

P06765

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.156 μg/mL-10 μg/mL

Sensitivity

0.039 μg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-97
1:2	Average %	95
	Range %	88-99
1:4	Average %	95
	Range %	91-102
1:8	Average %	95
	Range %	87-104

Recovery

The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-99
EDTA plasma (n=4)	97	89-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average	Corrected
10	2.684	2.617	2.651	2.463
5	2.204	2.186	2.195	2.007
2.5	1.563	1.608	1.586	1.398
1.25	0.881	0.899	0.890	0.702
0.625	0.480	0.491	0.486	0.298
0.312	0.345	0.337	0.341	0.153
0.156	0.247	0.250	0.249	0.061
0	0.185	0.190	0.188

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Rat PF4 ELISA Kit was designed for the quantitative measurement of Rat PF4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.

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Influence of brush length of PVP chains immobilized on silicon wafers on their blood compatibility Shengqiang Nie.et al,/,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.

Gene References into Functions

identified the informative Chronic Liver Allograft Dysfunction (CLAD)-associated genes; CXCL4, CXCR3, EGFR, JAK2, STAT3, and Collagen IV were associated with CLAD pathogenesis; validated that CXCL4 is upstream of these informative genes in the isolated hepatic stellate cells PMID:28053995
A carboxyl-terminal fragment of this protein effects a rapid histamine release from rat peritoneal mast cells. PMID:12383857
this study identified a novel regulatory element,TME(tandem repeat of MEIS1 binding element)in the PF4 promoter and demonstrated that MEIS1/PBX complexes bind to the TME PMID:12609849
PF4 promoters are transactivated by USF1 and 2 which may play an important role in megakaryocytic gene expression PMID:15187018
The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05). PMID:16797058