Rat Matrix Metalloproteinase 14,MMP-14 ELISA Kit

Promotion

Code: CSB-E13158r

Rate （N/A）
Review （0）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 46.88 pg/mL-3000 pg/mL

Sensitivity: 11.75 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

MMP-X1, MT1-MMP, MTMMP1, matrix metalloproteinase 14|matrix metalloproteinase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type matrix metalloproteinase 1|membrane-type-1 matrix m

Abbreviation

MMP14

Uniprot NO.

Q10739

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

46.88 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-102
1:4	Average %	97
	Range %	95-107
1:8	Average %	101
	Range %	96-105

Recovery

The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	98	95-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.288	2.289	2.289	2.092
1500	1.736	1.752	1.744	1.547
750	1.118	1.097	1.108	0.911
375	0.674	0.727	0.701	0.504
187.5	0.455	0.433	0.444	0.247
93.75	0.336	0.331	0.334	0.137
46.88	0.265	0.256	0.261	0.064
0	0.205	0.189	0.197

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Images

Rat Matrix Metalloproteinase 14,MMP-14 ELISA Kit

Description

This Rat MMP14 ELISA Kit was designed for the quantitative measurement of Rat MMP14 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Gene References into Functions

The results suggest that MMP-14 is involved in proliferative diabetic retinopathy angiogenesis. PMID:29853773
expression of MMP-14 was different between cardiac isografts and allografts. MMP-14 could interact with CD44 in allografts. PMID:27712978
that MT1-MMP contributes to intestinal development, particularly to villi morphogenesis PMID:27640084
Downregulation of miR-193a-3p promoted loss of type II collagen by directly targeting MMP14 in IDD. miR-193a-3p inhibited IDD in vitro and in vivo. miR-193a-3p may be a promising candidate for prevention of degenerative disc disease. PMID:26620678
The MMP-1 is present in several brain areas in normal conditions at a detectable level, and its expression increases after kainate-induced seizures. PMID:23683405
Hepatic stellate cells acquired dependence on collagen for their growth during the transition from quiescent to active states. The intramolecular RGD motifs of collagen were exposed by cleavage with their own membrane type 1 matrix metalloproteinase. PMID:24867951
High MT1-MMP is associated with glomerulosclerosis in diabetic nephropathy. PMID:21414971
The MT1-MMP expression was widespread in the dental follicle. PMID:21909755
MT1-MMP was found in the cytoplasm of cells that may remodel the gubernaculum for eversion. It provides the collagenolysis necessary for migration through the inguinal fat pad. Disruption of these processes may cause cryptorchidism. PMID:22152881
MT1-MMP plays a crucial role in RAGE-activated NADPH oxidase-dependent signaling pathways. PMID:20945382
Sympathetic neurons express and secrete MMP-2 and MT1-MMP to control nerve sprouting via pro-NGF conversion. PMID:20683769
Data suggest that MT1-MMP and TIMP-2 may have some role in cell proliferation during the eruption of the rat incisor tooth. PMID:20857180
Addition of follicle stimulating hormone to Sertoli cells increased the MMP-14 mRNA level three-fold, implicating a role in spermatogenesis. PMID:11928819
may play a role in extracellular matrix degradation during periodontitis PMID:12747454
cross-talk between the hemopexin domain of MT1-MMP and adjacent cell surface molecules is responsible for outside-in signaling. PMID:14729674
MT1-MMP is not only important in matrix degradation but also may affect the function of focal adhesions through FAK cleavage. PMID:15044209
expression patterns indicate that MT1-MMP may play key role in uterine postpartum involution and subsequent functional regenerative processes PMID:16740171
The differentiation-dependent co-expression of Mmp14 with EMMPRIN in the enamel organ and in odontoblasts indicates that EMMPRIN takes part in the induction of proteolytic enzymes in the rat tooth germ. PMID:17684756
These data indicate that Indian hedgehog promotes pancreatic stellate cell migration by enhancing brane-type 1 matrix metalloproteinase localization on the plasma membrane but is negatively regulated by Gli-1. PMID:18286538
MT1-MMP has multiple functions and is implicated in multiple steps of the differentiation process, acting to regulate homeostasis of the osteogenic differentiation. PMID:19027888