Rat Interleukin 5,IL-5 ELISA KIT

Product Details

Name: Rat Interleukin 5,IL-5 ELISA KIT
Brand: CUSABIO
SKU: CSB-E07435r

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Code: CSB-E07435r

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Citation （11）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Alternative Names

Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit

Abbreviation

IL5

Uniprot NO.

Q08125

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-91
1:2	Average %	92
	Range %	83-99
1:4	Average %	101
	Range %	93-107
1:8	Average %	96
	Range %	90-104

Recovery

The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-94
EDTA plasma (n=4)	94	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.407	2.457	2.432	2.268
500	1.768	1.736	1.752	1.588
250	1.065	1.098	1.082	0.918
125	0.704	0.735	0.720	0.556
62.5	0.448	0.469	0.459	0.295
31.2	0.323	0.300	0.312	0.148
15.6	0.221	0.229	0.225	0.061
0	0.161	0.167	0.164

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Rat Interleukin 5,IL-5 ELISA KIT

Description

Interleukin 5 is a cytokine that regulates eosinophil development, activation, and survival. As a member of the type I cytokine family, IL-5 is primarily produced by T helper 2 cells, mast cells, and eosinophils themselves. This glycoprotein acts as a key mediator in allergic inflammatory responses and parasitic infections. It promotes eosinophil differentiation from bone marrow precursors and enhances their recruitment to sites of inflammation. IL-5 signaling is particularly important in asthma, allergic rhinitis, and other eosinophil-associated disorders, making it a significant target for therapeutic intervention and biomarker research.

The Rat Interleukin 5,IL-5 ELISA KIT (CSB-E07435r) is designed for quantitative measurement of IL-5 in rat samples using a sandwich assay principle. This kit works with various sample types including serum, plasma, cell culture supernates, and tissue homogenates with a sample volume requirement of 50-100ul. The assay provides a detection range of 15.6 pg/mL to 1000 pg/mL with a sensitivity of 3.9 pg/mL. Results are measured at 450 nm wavelength. The complete assay can be performed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

Based on the provided information, no published research applications could be identified for this rat interleukin 5 ELISA kit from the available citation excerpts. Without specific research examples, a comprehensive applications summary cannot be generated at this time.

• Inflammatory research - Potential applications in studies examining eosinophil-mediated immune responses and allergic inflammation pathways
• Respiratory disease studies - Possible use in research examining asthma and other eosinophil-associated respiratory conditions
• Immunological profiling - Potential application in cytokine panel studies assessing immune system activation and regulation