Rat Interleukin 1α,IL-1α ELISA kit

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Name: Rat Interleukin 1α,IL-1α ELISA kit
Brand: CUSABIO
SKU: CSB-E04622r

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Code: CSB-E04622r

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Citation （6）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates.

Detect Range: 78 pg/ml-5000 pg/ml.

Sensitivity: 19.5 pg/ml.

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit

Abbreviation

IL1A

Uniprot NO.

P04822

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates.

Detection Range

78 pg/ml-5000 pg/ml.

Sensitivity

19.5 pg/ml.

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	98
	Range %	91-104
1:4	Average %	101
	Range %	98-107
1:8	Average %	89
	Range %	84-96

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

5000

2.657

2.556

2.607

2.425

2500

2.123

2.022

2.073

1.891

1250

1.468

1.447

1.458

1.276

625

0.927

0.916

0.922

0.740

312

0.568

0.579

0.574

0.392

156

0.415

0.404

0.410

0.228

0.312

0.300

0.306

0.124

0.180

0.183

0.182

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rat IL1A ELISA Kit was designed for the quantitative measurement of Rat IL1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Gene References into Functions

We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID:17455222

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Subcellular Location

Cytoplasm. Secreted.

Protein Families

IL-1 family

Database Links

UNIGENE: Ocu.2080

KEGG: ocu:100009250

STRING: 9986.ENSOCUP00000007573

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