Rat Interferon β,IFN-β/IFNB ELISA Kit

Product Details

Customer Reviews and Q&A

Citations

Target Background

Name: Rat Interferon β,IFN-β/IFNB ELISA Kit
Brand: CUSABIO
SKU: CSB-E04845r

Promotion

Code: CSB-E04845r

Rate （N/A）
Review （0）
Citation （2）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Alternative Names

Ifnb1 ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit

Abbreviation

IFNB1

Uniprot NO.

P70499

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	104
	Range %	98-110
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.516	2.587	2.552	2.380
500	2.021	2.106	2.064	1.892
250	1.362	1.324	1.343	1.171
125	0.761	0.739	0.750	0.578
62.5	0.503	0.534	0.519	0.347
31.25	0.375	0.359	0.367	0.195
15.6	0.276	0.295	0.286	0.114
0	0.174	0.170	0.172

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rat IFNB1 ELISA Kit was designed for the quantitative measurement of Rat IFNB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

■ Customer Reviews

Submit a Review here

■ Q&A

I will order IFN beta from you,
https://www.cusabio.com/Recombinant-Protein/Recombinant-Rat-Interferon-betaIfnb1-189.html
Could you guarantee I could measure that rat IFN beta with CSB-E04845r ELISA kit?
Is the two item compatible?

Thanks for your inquiry.
The standard of this kit CSB-E04845r is NS0-derived recombinant protein.
The recombinant proteins with different expression systems will be different. Sorry we didn't validate the detailed differences before, so we can not 100% guarantee that the kit could measure the protein.We suggest you do a pretest first.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Has antiviral, antibacterial and anticancer activities.

Gene References into Functions

results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID:26252165
IFN-gamma is able to activate rat intestinal mucous microvascular endothelial cells, thereby leading to upregulated expressions of IFN-alpha/beta. PMID:20214528
These findings validate IFN-beta as a therapeutic molecule for intraarticular gene therapy of arthritis. PMID:16984225
This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons. PMID:19541371