Rat Hemoglobin (Hb) ELISA Kit

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Code: CSB-E13036r
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, lysate for RBC.
Detect Range: 0.78 μg/mL-50 μg/mL
Sensitivity: 0.39 μg/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Target Name
hemoglobin, alpha 1
Alternative Names
Hba1 ELISA Kit; Hba-a1 ELISA Kit; Hemoglobin subunit alpha-1/2 ELISA Kit; Alpha-1/2-globin ELISA Kit; Hemoglobin alpha-1/2 chain ELISA Kit
Abbreviation
HBA1
Uniprot NO.
Species
Rattus norvegicus (Rat)
Sample Type
serum, plasma, lysate for RBC.
Detection Range
0.78 μg/mL-50 μg/mL
Sensitivity
0.39 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Competitive
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:5 Average % 101    
Range % 95-107    
1:10 Average % 93    
Range % 89-97    
1:20 Average % 95    
Range % 90-100    
1:40 Average % 90    
Range % 85-97    
Recovery
The recovery of rat Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 84-93    
EDTA plasma (n=4) 92 88-96    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
µg/ml OD1 OD2 Average      
50 0.169 0.176 0.1725      
25 0.292 0.311 0.3015      
12.5 0.486 0.483 0.4845      
6.25 0.819 0.795 0.807      
3.12 1.373 1.347 1.36      
1.56 2.013 2.024 2.0185      
0.78 2.461 2.493 2.477      
0 2.721 2.652 2.6865      
Troubleshooting and FAQs
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  • Rat Hemoglobin (Hb) ELISA Kit
Description

The rat hemoglobin (Hb) ELISA Kit is engineered for accurate measurement of rat hemoglobin levels from samples including serum, plasma, or lysate for RBC. It uses the Competitive-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the rat hemoglobin content in the sample. The color intensity is negatively correlated with hemoglobin content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

Hemoglobin is a two-way respiratory carrier, transporting oxygen from the lungs to the tissues and facilitating the return transport of carbon dioxide. In arterial circulation, hemoglobin has a high affinity for oxygen and a low affinity for carbon dioxide, organic phosphates, and hydrogen and chloride ions. In addition to being a molecular heat transducer through its oxygenation-deoxygenation cycle as well as a modulator of erythrocyte metabolism, hemoglobin's oxidation is an onset of erythrocyte senescence.It also mediates reactive oxygen and nitrogen species detoxification.p>

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
Involved in oxygen transport from the lung to the various peripheral tissues.
Gene References into Functions
  1. Results report that hemoglobin chains alpha2 nad beta are expressed in mammalian brain neurons and are regulated by a mitochondrial toxin. PMID:19479992

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Protein Families
Globin family
Tissue Specificity
Red blood cells.
Database Links
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