Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

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Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

Name: Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit
Brand: CUSABIO
SKU: CSB-E04570r

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Code: CSB-E04570r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 pg/ml - 1000 pg/ml

Sensitivity: 3.9 pg/ml

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit

Abbreviation

CSF2

Uniprot NO.

P48750

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 pg/ml - 1000 pg/ml

Sensitivity

3.9 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	97
	Range %	92-101
1:4	Average %	91
	Range %	86-95
1:8	Average %	103
	Range %	99-107

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.468

2.326

2.397

2.210

500

1.857

1.792

1.825

1.638

250

1.213

1.250

1.232

1.045

125

0.785

0.813

0.799

0.612

62.5

0.492

0.468

0.480

0.293

31.2

0.331

0.347

0.339

0.152

15.6

0.289

0.271

0.280

0.093

0.184

0.189

0.187

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rat CSF2 ELISA Kit was designed for the quantitative measurement of Rat CSF2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

■ Customer Reviews

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Nobiletin (NOB) nanoparticles ameliorate chronic paradoxical sleep deprivation (PSD)-induced cognitive deficits in rats Y Hu, D Hou, S Wang, N Wang, Q Wang, C Zhang,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Gene References into Functions

Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID:28550897
Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID:27364613
The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID:25677907
Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID:12071442
GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID:14557677
We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID:14651956
GM-CSF may play a significant role in follicular development PMID:15958400
GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID:16276414
GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID:16877635
GM-CSF acts as a neuroprotective protein in the CNS PMID:17457367
Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID:17478029
GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID:17953750
G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID:18832728
IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID:19022682