Rat Coagulation factor VII(F7) ELISA kit

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Code: CSB-EL007930RA

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.78 ng/mL-50 ng/mL

Sensitivity: 0.195 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Product Details

Alternative Names

F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit

Abbreviation

Uniprot NO.

Q8K3U6

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Blood Coagulation

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-98
1:400	Average %	97
	Range %	92-106
1:800	Average %	93
	Range %	88-99
1:1600	Average %	89
	Range %	83-95

Recovery

The recovery of rat F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	109	103-115

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.468	2.652	2.560	2.424
25	2.115	2.076	2.096	1.960
12.5	1.503	1.504	1.504	1.368
6.25	0.845	0.805	0.825	0.689
3.12	0.526	0.524	0.525	0.389
1.56	0.381	0.359	0.370	0.234
0.78	0.274	0.259	0.267	0.131
0	0.137	0.135	0.136

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Rat Coagulation factor VII(F7) ELISA kit

Description

This Rat Coagulation factor VII (F7) ELISA Kit was designed for the quantitative measurement of Rat Coagulation factor VII (F7) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

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Target Background

Function(From Uniprot)

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Gene References into Functions