Rat Cathepsin S(CTSS) ELISA kit

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Target Background

Name: Rat Cathepsin S(CTSS) ELISA kit
Brand: CUSABIO
SKU: CSB-EL006204RA

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Code: CSB-EL006204RA

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell lysates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

cathepsin S

Alternative Names

Ctss ELISA Kit; Cathepsin S ELISA Kit; EC 3.4.22.27 ELISA Kit

Abbreviation

CTSS

Uniprot NO.

Q02765

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	97
	Range %	93-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	94-102

Recovery

The recovery of rat CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-110
EDTA plasma (n=4)	84	80-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.563	2.457	2.510	2.395
500	1.997	2.076	2.037	1.922
250	1.378	1.404	1.391	1.276
125	0.877	0.821	0.849	0.734
62.5	0.567	0.554	0.561	0.446
31.25	0.358	0.331	0.345	0.230
15.6	0.227	0.240	0.234	0.119
0	0.113	0.117	0.115

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Rat Cathepsin S(CTSS) ELISA kit

Description

This Rat CTSS ELISA Kit was designed for the quantitative measurement of Rat CTSS protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.

Gene References into Functions

Findings demonstrate that neuronal reactive oxygen species promote maturation of microglial CatS which facilitates activation of NMDA in the spinal dorsal horn. PMID:28039043
Inhibition of cathepsin S release is one mechanism that is responsible for purinergic P2X7 antagonist efficacy in neuropathic pain. PMID:20629190
Activated rat hepatic stellate cells express the class II transactivator, the invariant chain (CD74), the MHC class II molecules, as well as cathepsin S, all of which are known to be responsible for the initial steps of successful antigen presentation. PMID:17178165
endogenous CatS released by peripheral macrophages contributes to the maintenance of neuropathic hyperalgesia following nerve injury PMID:17250968
There is an altered expression of cathepsin S and cathepsin X in the spinal cord of a neuropathic pain model; including cellular distribution and pain development. PMID:18700000
under neuropathic conditions, noxious stimulation of primary afferent fibers induces release of CatS from microglia, which liberates FKN from dorsal horn neurons, thereby contributing to the amplification and maintenance of chronic pain PMID:19474321

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Subcellular Location

Lysosome. Secreted. Cytoplasmic vesicle, phagosome.

Protein Families

Peptidase C1 family

Tissue Specificity

Highest levels occur in the ileum followed by spleen, brain, thyroid, ovary and uterus. Low levels are found in the liver, kidney, jejunum and lung with lowest levels in the heart.

Database Links

UNIGENE: Rn.11347

KEGG: rno:50654

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