Rat Antithrombin III(AT III)ELISA Kit

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Code: CSB-E13885r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 39 ng/mL-2500 ng/mL

Sensitivity: 9.75 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Product Details

Abbreviation

AT III

Uniprot NO.

Q5M7T5

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

39 ng/mL-2500 ng/mL

Sensitivity

9.75 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	104
	Range %	100-112
1:400	Average %	84
	Range %	80-88
1:800	Average %	90
	Range %	85-94
1:1600	Average %	97
	Range %	92-101

Recovery

The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	93	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
2500	2.466	2.312	2.389	2.251
1250	2.075	1.996	2.036	1.898
625	1.664	1.689	1.677	1.539
312.5	1.238	1.227	1.233	1.095
156	0.702	0.713	0.708	0.570
78	0.412	0.392	0.402	0.264
39	0.301	0.289	0.295	0.157
0	0.135	0.141	0.138

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat Antithrombin III (AT III) ELISA Kit is designed to measure Antithrombin-III (AT III) in rat serum, plasma, and tissue homogenates with remarkable accuracy and sensitivity.

With a detection range of 39 ng/mL-2500 ng/mL and a sensitivity of 9.75 ng/mL, this quantitative assay can detect even the smallest amount of AT III in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.

This kit is ideal for researchers investigating the role of AT III in cardiovascular diseases、anticoagulant system research. The sandwich measurement principle allows for accurate quantification of AT III in your samples, making it a valuable tool for any cardiovascular research project. This Rat Antithrombin III (AT III) ELISA Kit has been cited in 2 scientific publications, which speaks to its reliability and popularity within the research community.