Rat Alpha-fetoprotein,AFP ELISA Kit

Product Details

Name: Rat Alpha-fetoprotein,AFP ELISA Kit
Brand: CUSABIO
SKU: CSB-E08281r

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Code: CSB-E08281r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Alternative Names

AfpAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit

Abbreviation

AFP

Uniprot NO.

P02773

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	81-90
1:40	Average %	89
	Range %	82-94
1:80	Average %	101
	Range %	96-108
1:160	Average %	94
	Range %	89-99

Recovery

The recovery of rat AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	97	93-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.701	2.631	2.666	2.478
500	2.239	2.212	2.226	2.038
250	1.710	1.663	1.687	1.499
125	1.097	1.174	1.136	0.948
62.5	0.651	0.695	0.673	0.485
31.2	0.427	0.459	0.443	0.255
15.6	0.337	0.317	0.327	0.139
0	0.192	0.184	0.188

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rat AFP ELISA Kit was designed for the quantitative measurement of Rat AFP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .