Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit

Product Details

Customer Reviews and Q&A

Target Background

Name: Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit
Brand: CUSABIO
SKU: CSB-E09805Rb

Promotion

Code: CSB-E09805Rb

Rate （N/A）
Review （0）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 78 ng/mL-5000 ng/mL

Sensitivity: 19.5 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Alternative Names

APOBApolipoprotein B ELISA kit; Fragment ELISA kit

Abbreviation

Apo-B100

Uniprot NO.

P17165

Species

Oryctolagus cuniculus (Rabbit)

Sample Type

serum, plasma, tissue homogenates

Detection Range

78 ng/mL-5000 ng/mL

Sensitivity

19.5 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	90-102
1:200	Average %	92
	Range %	87-96
1:400	Average %	104
	Range %	98-108
1:800	Average %	85
	Range %	80-89

Recovery

The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	87	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.092	0.091	0.092
2500	0.146	0.138	0.142
1250	0.246	0.236	0.241
625	0.375	0.360	0.368
312	0.524	0.539	0.532
156	0.934	0.877	0.906
78	1.447	1.334	1.391
0	2.432	2.562	2.497

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rabbit Apo-B100 ELISA Kit was designed for the quantitative measurement of Rabbit Apo-B100 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

■ Customer Reviews

Submit a Review here

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Subcellular Location

Cytoplasm. Secreted. Lipid droplet.

Tissue Specificity

Detected in liver (at protein level). Highly expressed in liver. Detected at lower levels in jejunum, duodenum and kidney.

Database Links

UNIGENE: Ocu.1828

STRING: 9986.ENSOCUP00000004631

Promotions