Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit

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Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit

Name: Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit
Brand: CUSABIO
SKU: CSB-E15043Rb

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Code: CSB-E15043Rb

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 78 μU/mL-5000 μU/mL

Sensitivity: 19.5 μU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Target Name

Tartrate-resistant acid phosphatase 5b (TRAP5b)

Alternative Names

ACP5 ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit

Abbreviation

TRAP5b

Uniprot NO.

O97860

Species

Oryctolagus cuniculus (Rabbit)

Sample Type

serum, plasma, tissue homogenates

Detection Range

78 μU/mL-5000 μU/mL

Sensitivity

19.5 μU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TRAP5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-94
1:4	Average %	97
	Range %	87-101
1:8	Average %	91
	Range %	84-95

Recovery

The recovery of rabbit TRAP5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-96
EDTA plasma (n=4)	103	93-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μU/ml	OD1	OD2	Average	Corrected
5000	1.994	1.912	1.953	1.835
2500	1.776	1.745	1.761	1.643
1250	1.504	1.561	1.533	1.415
625	1.212	1.178	1.195	1.077
312	0.929	0.900	0.915	0.797
156	0.506	0.498	0.502	0.384
78	0.289	0.282	0.286	0.168
0	0.116	0.120	0.118

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rabbit TRAP5b ELISA Kit was designed for the quantitative measurement of Rabbit TRAP5b protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 μU/mL-5000 μU/mL and the sensitivity is 19.5 μU/mL.