Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

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Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

Name: Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit
Brand: CUSABIO
SKU: CSB-E14060Rb

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Code: CSB-E14060Rb

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell lysates

Detect Range: 1.25 ng/mL-80 ng/mL

Sensitivity: 0.31 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Alternative Names

BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit

Abbreviation

BGLAP

Uniprot NO.

P39056

Species

Oryctolagus cuniculus (Rabbit)

Sample Type

serum, plasma, tissue homogenates, cell lysates

Detection Range

1.25 ng/mL-80 ng/mL

Sensitivity

0.31 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-90
1:8	Average %	94
	Range %	86-98

Recovery

The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-94
EDTA plasma (n=4)	92	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.780	2.701	2.741	2.641
40	2.124	2.056	2.090	1.990
20	1.266	1.234	1.250	1.150
10	0.667	0.621	0.644	0.544
5	0.353	0.335	0.344	0.244
2.5	0.262	0.249	0.256	0.156
1.25	0.219	0.208	0.214	0.114
0	0.102	0.098	0.100

Troubleshooting and FAQs

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ELISA Data Analysis

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Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

Description

This Rabbit BGLAP ELISA Kit was designed for the quantitative measurement of Rabbit BGLAP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.