Pig intestinal fatty acid binding protein,iFABP ELISA Kit

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Pig intestinal fatty acid binding protein,iFABP ELISA Kit

Name: Pig intestinal fatty acid binding protein,iFABP ELISA Kit
Brand: CUSABIO
SKU: CSB-E13994p

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Code: CSB-E13994p

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma

Detect Range: 78 pg/mL-5000 pg/mL

Sensitivity: 19.5 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Name

fatty acid binding protein 2, intestinal

Alternative Names

FABP2 ELISA Kit; FABPIFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit

Abbreviation

FABP2

Uniprot NO.

Q45KW7

Species

Sus scrofa (Pig)

Sample Type

serum, plasma

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-93
1:2	Average %	100
	Range %	97-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	93
	Range %	90-96

Recovery

The recovery of pig iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	99	93-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.129	2.138	2.134	1.976
2500	1.628	1.656	1.642	1.484
1250	1.084	1.087	1.086	0.928
625	0.607	0.638	0.623	0.465
312	0.445	0.426	0.436	0.278
156	0.348	0.323	0.336	0.178
78	0.268	0.251	0.260	0.102
0	0.157	0.159	0.158

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Pig FABP2 ELISA Kit was designed for the quantitative measurement of Pig FABP2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

Gene References into Functions

The full-length cDNA of I-FABP was cloned from intestine and the mRNA was extensively present in various tissues, but I-FABP transcript of approximately 620 bp was more abundant in intestine than in other tissues. PMID:16529296
Plasma I-FABP concentration showed great variation within treatments, and no difference was observed in plasma I-FABP concentrations between the weaned conventionally and unweaned treatments PMID:18708596
Association analyses of the FABP2:g.412T>C with fatty acid composition traits were not significant in simple association. PMID:19207930

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Subcellular Location

Cytoplasm.

Protein Families

Calycin superfamily, Fatty-acid binding protein (FABP) family

Database Links

UNIGENE: Ssc.16525

KEGG: ssc:595106

STRING: 9823.ENSSSCP00000025576

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