Pig Insulin,INS ELISA Kit

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Name: Pig Insulin,INS ELISA Kit
Brand: CUSABIO
SKU: CSB-E06828p

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Code: CSB-E06828p

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Citation （9）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 2 μIU/mL-400 μIU/mL

Sensitivity: 1 μIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit

Abbreviation

INS

Uniprot NO.

P01315

Species

Sus scrofa (Pig)

Sample Type

serum, plasma, tissue homogenates

Detection Range

2 μIU/mL-400 μIU/mL

Sensitivity

1 μIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-105
1:2	Average %	93
	Range %	89-99
1:4	Average %	95
	Range %	89-101
1:8	Average %	95
	Range %	83-103

Recovery

The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	98	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
0	1.800	1.864	1.832
2	1.569	1.581	1.575
8	1.304	1.327	1.316
32	0.812	0.805	0.809
100	0.389	0.393	0.391
400	0.180	0.183	0.182

Troubleshooting and FAQs

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Pig Insulin,INS ELISA Kit

Description

Insulin is a peptide hormone produced by pancreatic beta cells that regulates glucose homeostasis by promoting cellular glucose uptake and glycogen synthesis. This hormone also affects lipid and protein metabolism. Its dysregulation leads to diabetes mellitus and other metabolic disorders. Insulin signaling pathways are fundamental to understanding energy metabolism, making it a key target for research in endocrinology, diabetes, and metabolic disease studies.

The Pig Insulin INS ELISA Kit (CSB-E06828p) is designed for quantitative measurement of insulin in Sus scrofa samples. This competitive assay accommodates serum, plasma, and tissue homogenates with a detection range of 2-400 μIU/mL and sensitivity of 1 μIU/mL. The assay requires 50-100 μL sample volume, operates at 450 nm detection wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in metabolic research studies to measure insulin concentrations in pig plasma and serum samples. The kit supports studies of glucose homeostasis and insulin resistance. Researchers use insulin measurements alongside other biochemical parameters to evaluate metabolic status and calculate insulin resistance indices.

• Metabolic profiling: Used to quantify plasma insulin levels as part of comprehensive biochemical parameter analysis including lipid profiles, glucose, and liver enzyme activities
• Insulin resistance evaluation: Applied in studies calculating homeostasis model assessment of insulin resistance using fasting insulin and glucose measurements
• Endocrine research: Used alongside other hormone measurements including growth hormone, progesterone, and insulin-like growth factor to evaluate hormonal profiles
• Nutritional studies: Applied to evaluate metabolic responses in dietary intervention research examining the effects of different nutritional treatments on insulin levels

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Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1伪 axis in skeletal muscle of growing pigs fed low-protein diets with varying branched Duan Y.et al,/,2017
Branched-chain amino acid ratios modulate lipid metabolism in adipose tissues of growing pigs Yehui Duan.et al,/,2018
β‐hydroxy‐β‐methyl butyrate promotes leucine metabolism and improves muscle fibre composition in growing pigs Yehui H. Duan.et al,/,2018
Preparation and characterization of insulin-loaded zein/carboxymethylated short-chain amylose complex nanoparticles Na Ji.et al,/,2018
Resveratrol Improves Hepatic Redox Status and Lipid Balance of Neonates with Intrauterine Growth Retardation in a Piglet Model K Cheng,/,2020
Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets M Dell'Anno,/,2021
Effect of Dietary Standardized Ileal Digestible Arginine to Lysine Ratio on Reproductive Performance, Plasma Biochemical Index, and Immunity of Gestating Sows X Wen, Z Jiang, X Yang, H Xiao, K Gao, L Wang,/,2024
Closed‐Loop Bioelectronic Artificial Pancreas Patch for Continuous Monitoring and Regulation of Blood Glucose in Diabetic Rats and Pigs Y Liu, C Zhang, L Xu, Y Ma, Y Ma, Y Chen,/,2025
Dietary Glycyl-Glutamine Supplementation Improves Growth, Immunity, Antioxidant Capacity, and Apparent Digestibility of Weaned Piglets X Jiang, D Li, M Chen, J Li, X Zhou, X Xiong, Y Yin,/,2025

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Gene References into Functions

We expect that these insulin-deficient pigs can be used in diabetes research to test the efficacy and safety of new drugs and the recipient of islet transplantation to investigate optimal transplantation strategies. PMID:29691708
Study used well-tempered bias exchange metadynamics simulations to determine the equilibrium ensembles of an insulin molecule under amyloidogenic conditions of low pH and high temperature. The folded state of a single insulin molecule was shown to be the most stable, longest-lived state even under amyloidogenic conditions. PMID:28445749
The findings are consistent with previous studies that indicate a link between Na,K-ATPase activity and SFK signaling. PMID:27748508
PTPLAD1 and AMPK are rapidly compartmentalized within the plasma membrane (PM) and Golgi/endosome fractions after insulin stimulation and that ATIC later accumulates in the Golgi/endosome fraction. PMID:25687571
Pdx-1, MafA and NeuroD1 bind to the A, C and E elements in the insulin promoter and regulate the transcriptional activity of the insulin promoter. PMID:24825179
The interplay of the adiponectin system, TNFalpha and insulin at a transcriptional level and, their effects on the adipogenic transcription factor PPARgamma, as well as on the activation of main insulin signaling pathways, is reported. PMID:24846393
Thermodynamics of insulin unfolding have been quantified by differential scanning calorimetry and thermal unfolding measurements to determine the extent and nature of their stabilization of the insulin hexamer. PMID:24811232
Exposing the hydrophobic core of insulin can induce the increase of amyloidogenicity and formation of higher-order polymerized fibrils, which is less toxic to membranes. PMID:24041697
Data suggest that a mutation in INS (C94Y) results a transgenic disease model for the investigation of permanent neonatal diabetes. PMID:23274907
The results show that modulation of plasma insulin levels by dietary carbohydrates seems possible in anabolic sows, but IGF-I levels are less easily modified. PMID:21635573
insulin increased GCLc promoter activity, which required a prerequisite increase or decrease in medium glucose PMID:21871559
SOCS3 is an important negative regulator of insulin signaling in porcine adipocytes. PMID:20683642
Plasma concentrations of insulin in pigs fed once per day were lower before feeding than after the meal. Plasma concentrations of insulin in ad libitum fed pigs exhibited random fluctuations. PMID:20399063
Data show that an expression cassette containing 1500bp of the porcine insulin promoter 5' UTR confers robust and specific transgene expression to beta-cells in vitro and in transgenic mice. PMID:19682540
Neutron diffraction data for T(6) porcine insulin were collected to 2.1 A resolution from a single crystal partly deuterated by exchange of mother liquor. PMID:19770501
computational analysis of the altered ionization of the B13 Glu in insulin B9 and B10 mutants of 4INS PMID:15326283
Results describe the in vitro folding/unfolding of insulin/single-chain insulin. PMID:16800793
A two-stage in vitro folding pathway of insulin is proposed, with six major folding intermediates captured during the folding process. PMID:17914645
Streptozotocin is able to impair in vitro neonatal pancreatic beta cell insulin release whereas human cytokines is not. PMID:17991146
A16Leu had much more significant effects on the foldability of insulin than B17Leu. PMID:18071885
These data demonstrated that feed allowance level has important consequence on embryo survival, embryo development and uterine development, possibly mediated by changes in concentrations of reproductive hormones. PMID:18700853
Functional surfaces of insulin were probed by photocross-linking of an extensive set of azido derivatives constructed by chemical synthesis. PMID:19321435